FHIT recombinant protein

FHIT, 1-147aa, Human, His tag, E Coli

Cat. Num. AAA203463

• Gene Names: FHIT; FRA3B; AP3Aase
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: FHIT; 1-147aa; Human; His tag; E Coli; Fragile histidine triad protein; Bis (5'-adenosyl)-triphosphatase; AP3Aase; Fragile histidine triad protein AP3A hydrolase; AP3A hydrolase fragile site 3p14.2; Bis 5' adenosyl triphosphatase; Diadenosine 5' 5''' P1 P3 triphosphate hydrolase; Dinucleosidetriphosphatase; FRA 3B; FRA3B; Fragile histidine triad; Fragile histidine triad gene; Tumor suppressor protein.; FHIT recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH
• Sequence Length: 147

• Applicable Applications for FHIT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for FHIT recombinant protein

FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for FHIT recombinant protein

Enzymes & Proteases

References

Kmiec Z., et al. (2009) J Physiol Pharmacol. 60 (4):63-70.; Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136 (3):333-50.;

NCBI and Uniprot Product Information

• NCBI GI #: 4503719
• NCBI GeneID: 2272
• NCBI Accession #: NP_002003
• NCBI GenBank Nucleotide #: NM_002012.3
• UniProt Accession #: P49789; Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9
• Molecular Weight: 17.9 kDa (155aa), confirmed by MALDI-TOF
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase isoform 1
• NCBI Official Synonym Full Names: fragile histidine triad
• NCBI Official Symbol: FHIT
• NCBI Official Synonym Symbols: FRA3B; AP3Aase
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: FHIT
• UniProt Synonym Gene Names: AP3Aase

NCBI Description

The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]

Uniprot Description

Cleaves P1-P3-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P1-P4-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P1-P3-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.