Rabbit TFIIIC102 Polyclonal Antibody | anti-TFIIIC102 antibody

TFIIIC102 (E131) Polyclonal Antibody

Cat. Num. AAA3002523

• Gene Names: GTF3C3; TFIIIC102; TFIIICgamma; TFiiiC2-102
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TFIIIC102; Polyclonal Antibody; TFIIIC102 (E131) Polyclonal Antibody; General transcription factor 3C polypeptide 3; Transcription factor IIIC 102 kDa subunit; TFIIIC 102 kDa subunit; Transcription factor IIIC subunit gamma; TF3C-gamma; GTF3C3; anti-TFIIIC102 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFIIIC102 (E131) polyclonal antibody detects endogenous levels of TFIIIC102 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 413

• Applicable Applications for anti-TFIIIC102 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human TFIIIC102.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TFIIIC102 (E131) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Testing Data

Related Product Information for anti-TFIIIC102 antibody

RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in malignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.

NCBI and Uniprot Product Information

• NCBI GI #: 332164729
• NCBI GeneID: 9330
• NCBI Accession #: NP_001193703.1
• NCBI GenBank Nucleotide #: NP_001193703.1
• UniProt Accession #: Q9Y5Q9
• Molecular Weight: ~ 101kDa
• NCBI Official Full Name: general transcription factor 3C polypeptide 3 isoform 2
• NCBI Official Synonym Full Names: general transcription factor IIIC subunit 3
• NCBI Official Symbol: GTF3C3
• NCBI Official Synonym Symbols: TFIIIC102; TFIIICgamma; TFiiiC2-102
• NCBI Protein Information: general transcription factor 3C polypeptide 3
• UniProt Protein Name: General transcription factor 3C polypeptide 3
• UniProt Gene Name: GTF3C3
• UniProt Synonym Gene Names: TFIIIC 102 kDa subunit; TFIIIC102; TF3C-gamma
• UniProt Entry Name: TF3C3_HUMAN

NCBI Description

The protein encoded by this gene is part of the TFIIIC2 complex, which binds to the promoters of small nuclear and cytoplasmic RNA genes in order to recruit RNA polymerase III. The TFIIIC2 complex is composed of six subunits. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

GTF3C3: Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: nucleoplasm; transcription factor TFIIIC complex

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase III promoter; transcription, DNA-dependent; 5S class rRNA transcription; gene expression; tRNA transcription from RNA polymerase III promoter