Rabbit anti-Human FGFRL1 Polyclonal Antibody | anti-FGFRL1 antibody

FGFRL1, NT (FGFRL1, FGFR5, FHFR, Fibroblast growth factor receptor-like 1, FGF homologous factor receptor, FGFR-like protein, Fibroblast growth factor receptor 5) (Azide free) (HRP)

Cat. Num. AAA6299191

• Gene Names: FGFRL1; FHFR; FGFR5
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FGFRL1; Polyclonal Antibody; NT (FGFRL1; FGFR5; FHFR; Fibroblast growth factor receptor-like 1; FGF homologous factor receptor; FGFR-like protein; Fibroblast growth factor receptor 5) (Azide free) (HRP); Fibroblast growth factor receptor 5; anti-FGFRL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FGFRL1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FGFRL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-168 amino acids from the N-terminal region of human FGFRL1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(FGFRL1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FGFRL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(FGFRL1 Antibody (N-term) western blot analysis in A549,HepG2,Y79 cell line lysates (35ug/lane).This demonstrates the FGFRL1 antibody detected the FGFRL1 protein (arrow).)

Related Product Information for anti-FGFRL1 antibody

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Product Categories/Family for anti-FGFRL1 antibody

Antibodies; Growth Factors; Cytokines

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). LopezJimenez, N., et al. Hum. Genet. 127(3):325-336(2010). Steinberg, F., et al. J. Biol. Chem. 285(3):2193-2202(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 51988914
• NCBI GeneID: 53834
• NCBI Accession #: NP_001004358.1
• NCBI GenBank Nucleotide #: NM_001004358.1
• UniProt Accession #: Q8N441; Q6PJN1; Q9BXN7; Q9H4D7; B2RAU9
• Molecular Weight: 54,537 Da
• NCBI Official Full Name: fibroblast growth factor receptor-like 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor-like 1
• NCBI Official Symbol: FGFRL1
• NCBI Official Synonym Symbols: FHFR; FGFR5
• NCBI Protein Information: fibroblast growth factor receptor-like 1; FGF homologous factor receptor; FGF receptor-like protein 1; FGFR-5; FGFR-like protein; fibroblast growth factor receptor 5
• UniProt Protein Name: Fibroblast growth factor receptor-like 1
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFRL1
• UniProt Synonym Gene Names: FGFR5; FHFR; FGF receptor-like protein 1; FGFR-5
• UniProt Entry Name: FGRL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. A marked difference between this gene product and the other family members is its lack of a cytoplasmic tyrosine kinase domain. The result is a transmembrane receptor that could interact with other family members and potentially inhibit signaling. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FGFRL1: Has a negative effect on cell proliferation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi apparatus; transport vesicle; plasma membrane; integral to membrane

Molecular Function: heparin binding; fibroblast growth factor binding; fibroblast growth factor receptor activity

Biological Process: negative regulation of cell proliferation; fibroblast growth factor receptor signaling pathway; protein heterooligomerization; skeletal development; protein homooligomerization

Disease: Wolf-hirschhorn Syndrome