Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of HL-60 cells using anti-IL22 antibody (M00963). Overlay histogram showing HL-60 cells stained with M00963 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IL22 Antibody (M00963, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Mouse anti-Human IL22 Monoclonal Antibody | anti-IL22 antibody
Anti-IL22 Antibody (monoclonal, 7F2)
Gene Names
IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Immunogen Affinity Purified
Synonyms
IL22; Monoclonal Antibody; Anti-IL22 Antibody (monoclonal; 7F2); Interleukin-22; IL-22; Cytokine Zcyto18; IL-10-related T-cell-derived-inducible factor; IL-TIF; ILTIF; ZCYTO18; UNQ3099/PRO10096; Interleukin 22; anti-IL22 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7F2
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
179
Applicable Applications for anti-IL22 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 0.1-0.5 mug/ml
FC/FACS: 1-3ug/1x106 cells
Tested Species: In-house tested species with positive results.
FC/FACS: 1-3ug/1x106 cells
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of HL-60 cells using anti-IL22 antibody (M00963). Overlay histogram showing HL-60 cells stained with M00963 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IL22 Antibody (M00963, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of HL-60 cells using anti-IL22 antibody (M00963). Overlay histogram showing HL-60 cells stained with M00963 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-IL22 Antibody (M00963, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Related Product Information for anti-IL22 antibody
Description: Mouse IgG monoclonal antibody for nmt55 p54nrb detection. Tested with WB, FCM in Human.
Background: Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Background: Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
References
1. Dumoutier, L., Van Roost, E., Ameye, G., Michaux, L., Renauld, J.-C. IL-TIF/IL-22: genomic organization and mapping of the human and mouse genes. Genes Immunity 1: 488-494, 2000. 2. Moore KW, de Waal Malefyt R, Coffman RL, O'Garra A (2001). "Interleukin-10 and the interleukin-10 receptor". Annu. Rev. Immunol. 19: 683-765.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,011 Da
NCBI Official Full Name
interleukin-22
NCBI Official Synonym Full Names
interleukin 22
NCBI Official Symbol
IL22
NCBI Official Synonym Symbols
TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
NCBI Protein Information
interleukin-22
UniProt Protein Name
Interleukin-22
Protein Family
UniProt Gene Name
IL22
UniProt Synonym Gene Names
ILTIF; ZCYTO18; IL-22; IL-TIF
NCBI Description
This gene is a member of the IL10 family of cytokines that mediate cellular inflammatory responses. The encoded protein functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. [provided by RefSeq, Jul 2018]
Uniprot Description
Cytokine that contributes to the inflammatory response in vivo.
Research Articles on IL22
Similar Products
Product Notes
The IL22 il22 (Catalog #AAA1752041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL22 Antibody (monoclonal, 7F2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 0.1-0.5 mug/ml FC/FACS: 1-3ug/1x106 cells Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the IL22 il22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL22, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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