Rabbit anti-Human FGFR4 Polyclonal Antibody | anti-FGFR4 antibody

FGFR4, NT (FGFR4, JTK2, TKF, Fibroblast growth factor receptor 4, CD334) (PE)

Cat. Num. AAA6299186

• Gene Names: FGFR4; TKF; JTK2; CD334
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified by Protein A/G Affinity Chromatography
• Synonyms: FGFR4; Polyclonal Antibody; NT (FGFR4; JTK2; TKF; Fibroblast growth factor receptor 4; CD334) (PE); CD334; anti-FGFR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGFR4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FGFR4 antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 24-55 in the N-terminal region of human FGFR4, UniProt Accession #P22455.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6012430. This demonstrates that MBS6012430 detected the FGFR4 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS6012430, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of WiDr cells using MBS6012430 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Fluorescent confocal image of HeLa cells stained withMBS6012430. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS6012430 (1:200, 2 hours at RT). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data)

Related Product Information for anti-FGFR4 antibody

FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Product Categories/Family for anti-FGFR4 antibody

Antibodies; CD Markers

References

1. Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). 2. Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). 3. Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). 4. Becker, N., et al., Cancer Epidemiol. Biomarkers Prev. 12(6):582-583 (2003). 5. Takenaka, H., et al., Arch. Dermatol. Res. 294(7):331-338 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 47524173
• NCBI GeneID: 2264
• NCBI Accession #: NP_002002.3
• NCBI GenBank Nucleotide #: NM_002011.3
• UniProt Accession #: P22455; O43785; Q14309; Q71TW8; Q8TDA0; AAB25788
• Molecular Weight: 87,954 Da
• NCBI Official Full Name: fibroblast growth factor receptor 4 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: FGFR4
• NCBI Official Synonym Symbols: TKF; JTK2; CD334
• NCBI Protein Information: fibroblast growth factor receptor 4; FGFR-4; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; tyrosine kinase related to fibroblast growth factor receptor
• UniProt Protein Name: Fibroblast growth factor receptor 4
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFR4
• UniProt Synonym Gene Names: JTK2; TKF; FGFR-4
• UniProt Entry Name: FGFR4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; TK group; FGFR family

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nucleoplasm; Golgi apparatus; transport vesicle; endoplasmic reticulum; integral to plasma membrane; cytoplasm; plasma membrane; extracellular region; intercellular junction; endosome

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; cell migration; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of lipid metabolic process; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; protein amino acid autophosphorylation; positive regulation of metalloenzyme activity; glucose homeostasis; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; phosphate ion homeostasis