AP1AR recombinant protein

AP1AR, 1-302aa, Human, His tag, E Coli

Cat. Num. AAA204802

• Gene Names: AP1AR; 2C18; GBAR; C4orf16; PRO0971; gamma-BAR
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: AP1AR; 1-302aa; Human; His tag; E Coli; Adaptor-related protein complex 1 associated regulatory protein; 2C18; C4orf16; gamma-BAR; GBAR; PRO0971; AP1AR recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDKK IQKELALQEE KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS ASDDSNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ TR
• Sequence Length: 302

• Applicable Applications for AP1AR recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AP1AR recombinant protein

Adaptor-related protein complex 1 associated regulatory protein, also known as AP1AR, is necessary for AP-1 dependent transport between the trans-Golgi network and endosomes. This protein regulates the membrane association of AP1G1/Gamma1-adaptin, one of the subunits of the AP-1 adapter complex. The direct interaction with AP1G1/Gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Recombinant human AP1AR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AP1AR recombinant protein

Signal Transduction

References

Neubrand V.E., et al. (2005) EMBO J. 24:1122-1133; Wollscheid B., et al. (2009) Nat. Biotechnol. 27:378-386;

NCBI and Uniprot Product Information

• NCBI GI #: 190358499
• NCBI GeneID: 55435
• NCBI Accession #: NP_061039
• NCBI GenBank Nucleotide #: NM_018569.5
• UniProt Accession #: Q63HQ0; Q96GG6; Q9H0V0; Q9P1L4; B2RCV7
• Molecular Weight: 36.4 kDa (322aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: AP-1 complex-associated regulatory protein isoform 1
• NCBI Official Synonym Full Names: adaptor related protein complex 1 associated regulatory protein
• NCBI Official Symbol: AP1AR
• NCBI Official Synonym Symbols: 2C18; GBAR; C4orf16; PRO0971; gamma-BAR
• NCBI Protein Information: AP-1 complex-associated regulatory protein
• UniProt Protein Name: AP-1 complex-associated regulatory protein
• Protein Family: AP-1 complex-associated regulatory protein
• UniProt Gene Name: AP1AR
• UniProt Synonym Gene Names: C4orf16; GBAR; Gamma-BAR; Gadkin

Uniprot Description

Necessary for adaptor protein complex 1 (AP-1)-dependent transport between the trans-Golgi network and endosomes. Regulates the membrane association of AP1G1/gamma1-adaptin, one of the subunits of the AP-1 adaptor complex. The direct interaction with AP1G1/gamma1-adaptin attenuates the release of the AP-1 complex from membranes. Regulates endosomal membrane traffic via association with AP-1 and KIF5B thus linking kinesin-based plus-end-directed microtubular transport to AP-1-dependent membrane traffic. May act as effector of AP-1 in calcium-induced endo-lysosome secretion. Inhibits Arp2/3 complex function; negatively regulates cell spreading, size and motility via intracellular sequestration of the Arp2/3 complex.