Western Blot (WB)
Rabbit anti-Cat FEL D1 Polyclonal Antibody | anti-FEL D1 antibody
FEL D1 Major Allergen I Polypeptide Chain 1 Antibody Affinity Purified
Gene Names
LOC677877; CH1
Reactivity
Cat
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
FEL D1; Polyclonal Antibody; FEL D1 Major Allergen I Polypeptide Chain 1 Antibody Affinity Purified; CH1; Fel D1; AG4; Allergen Cat-1; Allergen D I-A; Allergen FDI; Allergen Fel Di; Allregen: Fel d 1-A; anti-FEL D1 antibody
Host
Rabbit
Reactivity
Cat
Clonality
Polyclonal
Form/Format
Affinity Purified Immunoglobulins
Concentration
0.65-0.75 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
92
Applicable Applications for anti-FEL D1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 1-50 on feline Major Cat Allergen I polypeptide chain 1.
Expression
Saliva and sebaceous glands.
Determinant
N-epitope
Structure
Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.
Subcellular Location
Secreted; Extracellular region
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-FEL D1 antibody
A number of house pet bourn allergens have been studied for allergic reactions in general population. Domestic cat (Felis domestica) is an important source of indoor allergen Fel D1 that affects a large population. Fel D1 is also established to cause catinduce asthma in sensitive patients. The levels of Fel D1 and Can F1 were high in homes of former pet-owners compared to the homes without pets. Children with mild allergy to animal dander may have profound increase in bronchial sensitivity (increased sputum eosinophil peroxidase (EPO) and myeloperoxidase (MPO) activity as a result of constant exposure of animal allergens (Fel D 1 and Can F1) at public places. Allergic reactions to Fel D1 are effectively reduced by conventional immunotherapy in many patients, but this type of immunotherapy has severe reactions, including anaphylaxis and requires several years of injections for successful desensitization. Several strategies have been emploved to address Hlese issues Including use of cat allerge'1 peptldes. Chimeric constructs of Fel D1 and Fc portion of human IgEs (1). The synthetic cat allergen peptides lack the tertiary structure of native Fel D1 and posses reduced capacity to bind Fel D1-speciflc IgE. These peptides when given to patients sensitive to cat allergens showed improvement in pulmonary function and tolerance to cats (1) The exposure of chimeric human -cat fusion protein composed of truncated human IgGFcgamma1 and the Fel D1. The chimeriC protein induces dose dependent inhibition of FelD1-driven IgE mediated histamine release form cat allergic donors (2). The allergen is a 38 kDa dimer composed of two 19 kDa subunits. Each 19 kDa subunit comprises two disulfide linked polypeptide chains, a light alpha-chain and a heavy beta-chain containing an N-linked oligosaccharide. The alpha light chain and the heavy beta
chain are linked in an anti-parallel configuration. The Fel D1 glycosylation at Asn 33 is of heterogeneous triantennary structure caused by terminal sialic acid and fructose residues attached to beta-galactose residues.
Anti-Fel D1-selective antibodies are developed against 3 epitopes covering the majority of the protein. The affinity purified mono-specific polyclonal antibodies to FEL D1 strongly labels a 15-18 kDa protein in cat saliva samples examined. A cyclic peptide methodology was employed for generating antibodies, which results in higher titer and specificity. Western blot positive control in ready-to-use SDS-sample buffer (MBS542727) and antigenic blocking peptide (MBS543668) may be available upon request. Antibodies can be conjugated with fluorescent probes or other secondary enzymes, please enquire.
Anti-Fel D1-selective antibodies are developed against 3 epitopes covering the majority of the protein. The affinity purified mono-specific polyclonal antibodies to FEL D1 strongly labels a 15-18 kDa protein in cat saliva samples examined. A cyclic peptide methodology was employed for generating antibodies, which results in higher titer and specificity. Western blot positive control in ready-to-use SDS-sample buffer (MBS542727) and antigenic blocking peptide (MBS543668) may be available upon request. Antibodies can be conjugated with fluorescent probes or other secondary enzymes, please enquire.
Product Categories/Family for anti-FEL D1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Major allergen I polypeptide chain 1
NCBI Official Symbol
LOC677877
NCBI Official Synonym Symbols
CH1
NCBI Protein Information
major allergen I polypeptide chain 1
UniProt Protein Name
Major allergen I polypeptide chain 1
UniProt Gene Name
CH1
UniProt Synonym Gene Names
Allergen FdI; Allergen Fel dI
UniProt Entry Name
FEL1A_FELCA
Research Articles on FEL D1
Similar Products
Product Notes
The FEL D1 ch1 (Catalog #AAA540680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FEL D1 Major Allergen I Polypeptide Chain 1 Antibody Affinity Purified reacts with Cat and may cross-react with other species as described in the data sheet. AAA Biotech's FEL D1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the FEL D1 ch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FEL D1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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