Western Blot (WB)
(Western Blot of FEL D1 antibody (FELD1-121AP) with:1) Control Cat Saliva2) Mutated Cat Salive60 ul of sample was applied to each lane. Apparent MW is 11 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. )
FEL D1 blocking peptide
FEL D1 Major Allergen I Polypeptide Chain 1 Antigenic Blocking Peptide C-epitope
Gene Names
LOC677877; CH1
Applications
Immunodepletion, Immunocompetition
Synonyms
FEL D1; FEL D1 Major Allergen I Polypeptide Chain 1 Antigenic Blocking Peptide C-epitope; H1; Fel D1; AG4; Allergen Cat-1; Allergen D I-A; Allergen FDI; Allergen Fel Di; Allregen: Fel d 1-A; FEL D1 blocking peptide
Form/Format
Antigenic Blocking Peptide
Sequence Length
92
Applicable Applications for FEL D1 blocking peptide
Immunodepletion, Immunocompetition
Application Notes
Immunohistohcemistry 1:250
Immunofluorescence: 1:250
Immunohistochemistry: 1:250
Immunoprecipitation: 1:250
Western Blot: 1:500
Immunofluorescence: 1:250
Immunohistochemistry: 1:250
Immunoprecipitation: 1:250
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 42-88 on feline Major Cat Allergen I polypeptide chain 1.
Preparation and Storage
Store at -20°C for long term storage.
Western Blot (WB)
(Western Blot of FEL D1 antibody (FELD1-121AP) with:1) Control Cat Saliva2) Mutated Cat Salive60 ul of sample was applied to each lane. Apparent MW is 11 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. )
Western Blot (WB)
(Western Blot of FEL D1 antibody (FELD1-121AP) with:1) Control Cat Saliva2) Mutated Cat Salive60 ul of sample was applied to each lane. Apparent MW is 11 kDa.Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. )
Related Product Information for FEL D1 blocking peptide
A number of house pet bourn allergens have been studied for allergic reactions in general population. Domestic cat (Felis domestica) is an important source of indoor allergen Fel D1 that affects a large opulation. Fel Dl is also established to cause catinduce asthma in sensitive patients. The levels of Fel D1 nd Can Fl were high in homes of former pet-owners compared to the homes without pets. Children with ild allergy to animal dander may have profound increase in bronchial sensitivity (increased sputum osinophil peroxidase (EPO) and myeloperoxidase (MPO) activity as a result of constant exposure of nimal allergens (Fel D1 and Can Fl) at public places. Allergic reactions to Fel D1 are effectively reduced y conventional immunotherapy in many patients, but this type of irnmunotherapy has severe reactions, ncluding anaphylaxis and requires several years of injections for successful desensitization. Several trategies have been employed to address these issues including use of cat allergen peptides, chimeric onstructs of Fel D1 and Fc portion of human lgEs (1). The synthetic cat allergen peptides lack the tertiary tructure of native Fel D1 and posses reduced capacity to bind Fel D1-specific lgE. These peptides when iven to patients sensitive to cat allergens showed improvement in pulmonary function and tolerance to ats (1). The exposure of chimeric human -cat fusion protein composed of truncated human lgGFcgammal nd the Fel Dl . The chimeric protein induces dose dependent inhibition of Fel D1-driven lgE mediated istamine release form cat allergic donors (2). The allergen is a 38 kDa dimer composed of two 19 kDa subunits. ach 19 kDa subunit comprises two disulfide linked polypeptide chains, a light alpha-chain and a heavy beta-chain containing an N-linked ligosaccharide. The alpha light chain and the heavy beta chain are linked in an anti-parallel configuration. The Fel Dl glycosylation at Asn 3 is of heterogeneous triantennary structure caused by terminal sialic acid and fructose residues attached to beta-galactose residues. FabGennix has developed anti-Fel D1-selective antibodies against 3 epitopes covering the majority of the protein. The affinity purified ono- pecific polyclonal antibodies to FEL D1 strongly labels a 15-18 kDa protein in cat saliva samples examined. MBS employs cyclic eptide methodology for generating antibodies, which results in higher titer and specificity. Western blot positive control in readyto-use DS-sample buffer (PC-FELDI ) and antigenic blocking peptide (P-FELD1 .3) are available. Antibodies can be conjugated with fluorescent robes or other secondary enzymes upon request at extra charge.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Major allergen I polypeptide chain 1
NCBI Official Symbol
LOC677877
NCBI Official Synonym Symbols
CH1
NCBI Protein Information
major allergen I polypeptide chain 1
UniProt Protein Name
Major allergen I polypeptide chain 1
UniProt Gene Name
CH1
UniProt Synonym Gene Names
Allergen FdI; Allergen Fel dI
UniProt Entry Name
FEL1A_FELCA
Research Articles on FEL D1
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Product Notes
The FEL D1 ch1 (Catalog #AAA543670) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FEL D1 can be used in a range of immunoassay formats including, but not limited to, Immunodepletion, Immunocompetition. Immunohistohcemistry 1:250 Immunofluorescence: 1:250 Immunohistochemistry: 1:250 Immunoprecipitation: 1:250 Western Blot: 1:500. Researchers should empirically determine the suitability of the FEL D1 ch1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FEL D1, Blocking Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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