FE65 Polyclonal Antibody | anti-FE65 antibody

Anti-FE65 Antibody

Cat. Num. AAA178400

• Gene Names: APBB1; RIR; FE65; MGC:9072
• Reactivity: Mouse; Predicted to work with: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: FE65; Polyclonal Antibody; Anti-FE65 Antibody; Amyloid beta A4 precursor protein-binding family B member 1; Adaptor protein FE65a2; Amyloid beta (A4) precursor protein binding family B member 1; Amyloid Beta A4 Precursor Protein Binding Family B; Amyloid beta A4 precursor protein binding family B member 1; Amyloid beta precursor protein binding family B member 1; APBB 1; APBB1; APBB1_HUMAN; FE 65; Fe65 protein; Protein Fe65; RIR; stat like protein; amyloid beta (A4) precursor protein-binding; family B; member 1 (Fe65); anti-FE65 antibody

• Reactivity: Mouse; Predicted to work with: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• Sequence Length: 710

• Applicable Applications for anti-FE65 antibody: Western Blot (WB)
• Application Notes: Western Blot; ; Concentration: 0.1-0.5ug/ml; Tested Species: Ms; Predicted Species: Hu; ; WB: The detection limit for FE65 is approximately 0.1 ng/lane under reducing conditions.; ; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applications have not been tested. ; ; Optimal dilutions should be determined by end users.
• Immunogen: E Coli-derived human FE65 recombinant protein (Position: Q295-A613). Human FE65 shares 95%and 96% amino acid (aa) sequence identity with mouse and rat FE65, respectively.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. ; ; Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- FE65 antibody, Western blottingAll lanes: Anti at 0.5ug/mlWB: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 77KDObserved bind size: 77KD)

Related Product Information for anti-FE65 antibody

Description: Rabbit IgG polyclonal antibody for Amyloid beta A4 precursor protein-binding family B member 1(APBB1) detection. Tested with WB in Human;Mouse.

Background: APBB1 is also known as RIR or FE65. The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

References

1. Chen GB, et al. Association of amyloid precursor protein-binding protein, family B, member 1 with nicotine dependence in African and European American smokers. Hum Genet, 2008 Nov. 2. Cheung HN, et al. FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth. FASEB J, 2014 Jan. 3. Ward MW, et al. The amyloid precursor protein intracellular domain(AICD) disrupts actin dynamics and mitochondrial bioenergetics. J Neurochem, 2010 Apr.

NCBI and Uniprot Product Information

• NCBI GI #: 4502131
• NCBI GeneID: 322
• NCBI Accession #: NP_001155.1
• NCBI GenBank Nucleotide #: NM_001164.4
• UniProt Accession #: O00213; Q7Z324; Q96A93; A1E379; A6NH82; A6NL69; B7Z1J5; B7Z1J6; B7Z2Y0; D3DQT2; V9GYK0; V9GYT4
• NCBI Official Full Name: amyloid beta A4 protein-binding family B member 1 isoform a
• NCBI Official Synonym Full Names: amyloid beta precursor protein binding family B member 1
• NCBI Official Symbol: APBB1
• NCBI Official Synonym Symbols: RIR; FE65; MGC:9072
• NCBI Protein Information: amyloid beta A4 precursor protein-binding family B member 1
• UniProt Protein Name: Amyloid beta A4 precursor protein-binding family B member 1
• UniProt Gene Name: APBB1
• UniProt Synonym Gene Names: FE65; RIR
• UniProt Entry Name: APBB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

Fe65: adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on Y142 (H2AXpY142) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as TIP60, probably explains its trancription activation activity.

Protein type: Adaptor/scaffold; Transcription regulation; Apoptosis

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cell soma; cytoplasm; dendritic spine; growth cone; lamellipodium; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; protein complex; synapse

Molecular Function: beta-amyloid binding; chromatin binding; histone binding; protein binding; protein complex binding; tau protein binding; transcription factor binding

Biological Process: apoptosis; axonogenesis; cell cycle arrest; double-strand break repair; negative regulation of cell growth; negative regulation of thymidylate synthase biosynthetic process; positive regulation of apoptosis; positive regulation of DNA repair; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to iron ion; signal transduction; transcription, DNA-dependent