Rabbit APBB1 Polyclonal Antibody | anti-APBB1 antibody

APBB1 Antibody

Cat. Num. AAA2517450

• Gene Names: APBB1; RIR; FE65; MGC:9072
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antigen affinity purification
• Synonyms: APBB1; Polyclonal Antibody; APBB1 Antibody; RIR; FE65; MGC:9072; anti-APBB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 710

• Applicable Applications for anti-APBB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant protein of human APBB1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using APBB1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using APBB1 antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using APBB1 antibody at dilution of 1:100 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using APBB1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-APBB1 antibody

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 12229629
• NCBI GeneID: 322
• Molecular Weight: 52,656 Da
• NCBI Official Full Name: Amyloid beta A4 protein-binding family B member 1
• NCBI Official Synonym Full Names: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
• NCBI Official Symbol: APBB1
• NCBI Official Synonym Symbols: RIR; FE65; MGC:9072
• NCBI Protein Information: amyloid beta A4 precursor protein-binding family B member 1; adaptor protein FE65a2; stat-like protein
• UniProt Protein Name: Amyloid beta A4 precursor protein-binding family B member 1
• Protein Family: Amyloid beta A4 precursor protein-binding family
• UniProt Gene Name: APBB1
• UniProt Synonym Gene Names: FE65; RIR
• UniProt Entry Name: APBB1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

Fe65: adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on Y142 (H2AXpY142) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as TIP60, probably explains its trancription activation activity.

Protein type: Adaptor/scaffold; Transcription regulation; Apoptosis

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: growth cone; lamellipodium; cytoplasm; plasma membrane; synapse; nuclear speck; nucleus

Molecular Function: protein binding; histone binding; beta-amyloid binding; chromatin binding; transcription factor binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; positive regulation of DNA repair; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; neuron migration; signal transduction; negative regulation of thymidylate synthase biosynthetic process; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; axonogenesis; double-strand break repair; visual learning; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; cell cycle arrest; response to DNA damage stimulus