Rabbit anti-Human, Mouse FBXW8 Polyclonal Antibody | anti-FBXW8 antibody

FBXW8, ID (FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6, F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29) (Biotin)

Cat. Num. AAA6298562

• Gene Names: FBXW8; FBW6; FBW8; FBX29; FBXW6; FBXO29
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXW8; Polyclonal Antibody; ID (FBXW8; FBW6; FBW8; FBX29; FBXO29; FBXW6; F-box/WD repeat-containing protein 8; F-box and WD-40 domain-containing protein 8; F-box only protein 29) (Biotin); F-box only protein 29; anti-FBXW8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FBXW8 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FBXW8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-303 amino acids from the Central region of human FBXW8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXW8 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the FBXW8 antibody detected the FBXW8 protein (arrow).)

Western Blot (WB)

(FBXW8 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the FBXW8 antibody detected the FBXW8 protein (arrow).)

Immunohistochemistry (IHC)

(FBXW8 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXW8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FBXW8 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FBXW8 antibody

Antibodies; WD Repeat Proteins

References

Tsutsumi, T., et al. Mol. Cell. Biol. 28(2):743-751(2008). Koch, H.B., et al. Cell Cycle 6(2):205-217(2007). Okabe, H., et al. PLoS ONE 1, E128 (2006): Watanabe, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424(2004). Dias, D.C., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16601-16606(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 30795120
• NCBI GeneID: 26259
• NCBI Accession #: NP_036306.1
• NCBI GenBank Nucleotide #: NM_012174
• UniProt Accession #: Q8N3Y1; Q9UK95
• Molecular Weight: 60,705 Da
• NCBI Official Full Name: Homo sapiens F-box and WD repeat domain containing 8 (FBXW8), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: F-box and WD repeat domain containing 8
• NCBI Official Symbol: FBXW8
• NCBI Official Synonym Symbols: FBW6; FBW8; FBX29; FBXW6; FBXO29
• NCBI Protein Information: F-box/WD repeat-containing protein 8; F-box and WD-40 domain protein 8; F-box and WD-40 domain-containing protein 8; F-box only protein 29
• UniProt Protein Name: F-box/WD repeat-containing protein 8
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: FBXW8
• UniProt Synonym Gene Names: FBW6; FBW8; FBX29; FBXO29; FBXW6
• UniProt Entry Name: FBXW8_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXW8: Substrate-recognition component of a SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Cell development/differentiation

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; SCF ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity

Biological Process: cell proliferation; protein ubiquitination; positive regulation of dendrite morphogenesis; Golgi organization and biogenesis