Mouse anti-Human CSF1R Monoclonal Antibody | anti-CSF1R antibody

Anti-CSF1R Mouse mAb

Cat. Num. AAA476501

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CSF1R; Monoclonal Antibody; Anti-CSF1R Mouse mAb; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; anti-CSF1R antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 6B9H2
• Form/Format: Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
• Sequence Length: 454

• Applicable Applications for anti-CSF1R antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human CSF1R (AA: 344-497) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using CSF1R mAb against human CSF1R (AA)

Western Blot (WB)

(Western blot analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using CSF1R mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CSF1R mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CSF1R mouse mAb with DAB staining.)

Related Product Information for anti-CSF1R antibody

Entrez Summary: The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction.

NCBI and Uniprot Product Information

• NCBI GI #: 125858971
• NCBI GeneID: 1436
• Molecular Weight: 108
• NCBI Official Full Name: CSF1R protein, partial
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• Protein Family: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: cytokine binding; macrophage colony stimulating factor receptor activity; protein homodimerization activity

Biological Process: cell proliferation; cytokine and chemokine mediated signaling pathway; hemopoiesis; inflammatory response; intercellular junction maintenance; macrophage differentiation; monocyte differentiation; multicellular organismal development; osteoclast differentiation; peptidyl-tyrosine phosphorylation; phosphatidylinositol metabolic process; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid autophosphorylation; regulation of bone resorption; regulation of cell shape; ruffle organization and biogenesis; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids