Rabbit anti-Human FBXO6 Polyclonal Antibody | anti-FBXO6 antibody

FBXO6 Antibody

Cat. Num. AAA7043973

• Gene Names: FBXO6; FBG2; FBS2; FBX6; Fbx6b
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: FBXO6; Polyclonal Antibody; FBXO6 Antibody; F-box only protein 6; F-box protein that recognizes sugar chains 2; F-box/G-domain protein 2; FBG2; FBS2; FBX6; anti-FBXO6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 293

• Applicable Applications for anti-FBXO6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human F-box only protein 6 protein (C-190AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-101870 / sc-99108
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: FBXO6 antibody at 3.87 ug/ml+ A431 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 34 kDaObserved band size: 34120 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7043973 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7043973 at dilution of 1:100)

Related Product Information for anti-FBXO6 antibody

Substrate-recognition component of some SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.

NCBI and Uniprot Product Information

• NCBI GI #: 8922188
• NCBI GeneID: 26270
• NCBI Accession #: NP_060908.1
• NCBI GenBank Nucleotide #: NM_018438.5
• UniProt Accession #: Q9NRD1; Q9UKT3; B1AK42; B2RC88
• Molecular Weight: 33,933 Da
• NCBI Official Full Name: F-box only protein 6
• NCBI Official Synonym Full Names: F-box protein 6
• NCBI Official Symbol: FBXO6
• NCBI Official Synonym Symbols: FBG2; FBS2; FBX6; Fbx6b
• NCBI Protein Information: F-box only protein 6
• UniProt Protein Name: F-box only protein 6
• UniProt Gene Name: FBXO6
• UniProt Synonym Gene Names: FBG2; FBS2; FBX6
• UniProt Entry Name: FBX6_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class, and its C-terminal region is highly similar to that of rat NFB42 (neural F Box 42 kDa) which may be involved in the control of the cell cycle. [provided by RefSeq, Jul 2008]

Uniprot Description

FBXO6: Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex- type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytoplasm; cytosol; SCF ubiquitin ligase complex

Molecular Function: glycoprotein binding; protein binding; ubiquitin-protein ligase activity

Biological Process: DNA damage checkpoint; DNA repair; ER-associated protein catabolic process; protein polyubiquitination; proteolysis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process