Rabbit anti-Human FBXO3 Polyclonal Antibody | anti-FBXO3 antibody

FBXO3, CT (FBXO3, FBX3, F-box only protein 3) (AP)

Cat. Num. AAA6298395

• Gene Names: FBXO3; FBA; FBX3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FBXO3; Polyclonal Antibody; CT (FBXO3; FBX3; F-box only protein 3) (AP); F-box only protein 3; anti-FBXO3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FBXO3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FBXO3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 395-422 in the C-terminal region of human FBXO3, UniProt Accession #Q9UK99. Species sequence homology: bovine, mouse, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of HepG2 cell line lysates (35ug/lane) usingMBS646919. FBXO3 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram) usingMBS646919. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FBXO3 antibody

Antibodies; Ubiquitin Ligases

References

Shima, Y., et.al., Mol. Cell. Biol. 28 (23), 7126-7138 (2008). Ilyin, G.P., et.al., Genomics 67 (1), 40-47 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 71297179
• NCBI GeneID: 26273
• UniProt Accession #: Q9UK99; Q86X90; Q9H0V2; Q9NUX2; B3KY16; D3DR05
• Molecular Weight: 54,561 Da
• NCBI Official Full Name: FBXO3 protein
• NCBI Official Synonym Full Names: F-box protein 3
• NCBI Official Symbol: FBXO3
• NCBI Official Synonym Symbols: FBA; FBX3
• NCBI Protein Information: F-box only protein 3; F-box protein FBX3
• UniProt Protein Name: F-box only protein 3
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO3
• UniProt Synonym Gene Names: FBX3
• UniProt Entry Name: FBX3_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Ref.6

Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex. Ref.6

Subcellular location: Nucleus. Note: Colocalizes with PML at the peripheries of nuclear bodies.

Sequence similarities: Contains 1 apaG domain.Contains 1 F-box domain.