Western Blot (WB)
(Host: MouseTarget Name: STK3Sample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)
Rabbit STK3 Polyclonal Antibody | anti-STK3 antibody
STK3 antibody - N-terminal region
Western Blot (WB)
(Host: MouseTarget Name: STK3Sample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: MouseTarget Name: STK3Sample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Lanes:Lane 1: 30ug of HeLa cell lysateLane 2: 30ug of 293T cell lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Jixin Dong & Yuanhong Chen,University of Nebraska)
Western Blot (WB)
(Lanes:Lane 1: 30ug of HeLa cell lysateLane 2: 30ug of 293T cell lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Jixin Dong & Yuanhong Chen,University of Nebraska)
Western Blot (WB)
(Lanes:Lane1: 100ug untransfected COS-7 lysateLane2: 100ug mock transfected Cos-7 lysateLane3: 100ug STK3 transfected Cos-7 lysateLane4: 50 ug STK3 transfected Cos-7 lysateLane5: 25 ug STK3 transfected Cos-7 lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Anonymous)
Western Blot (WB)
(Lanes:Lane1: 100ug untransfected COS-7 lysateLane2: 100ug mock transfected Cos-7 lysateLane3: 100ug STK3 transfected Cos-7 lysateLane4: 50 ug STK3 transfected Cos-7 lysateLane5: 25 ug STK3 transfected Cos-7 lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Anonymous)
Western Blot (WB)
(Lanes:Lane1: 100ug untransfected RPE-1 lysateLane2: 100ug untransfected RPE-1 lysateLane3: 100ug STK3 induced RPE-1 lysateLane4: 100ug STK3 induced RPE-1 lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Anonymous)
Western Blot (WB)
(Lanes:Lane1: 100ug untransfected RPE-1 lysateLane2: 100ug untransfected RPE-1 lysateLane3: 100ug STK3 induced RPE-1 lysateLane4: 100ug STK3 induced RPE-1 lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:5000Gene Name:STK3Submitted by:Anonymous)
Western Blot (WB)
(WB Suggested Anti-STK3 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)
Western Blot (WB)
(WB Suggested Anti-STK3 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)
Target Description: Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand.Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-11 U26424.1 1-11 12-2826 BC010640.2 1-2815
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.
Protein type: Autophagy; EC 2.7.11.1; Kinase, protein; MST subfamily; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: cytoplasm; cytosol; protein complex
Molecular Function: ATP binding; magnesium ion binding; protein binding; protein dimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein binding; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization; signal transduction
Research Articles on STK3
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Product Notes
The STK3 stk3 (Catalog #AAA3209126) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK3 antibody - N-terminal region reacts with Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish (Tested Reactivity: Human, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's STK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the STK3 stk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: MEQPPAPKSK LKKLSEDSLT KQPEEVFDVL EKLGEGSYGS VFKAIHKESG. It is sometimes possible for the material contained within the vial of "STK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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