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Western Blot (WB) (Western blot analysis of extracts from PANC-1 CELLS, using FBXO27 Antibody. The lane on the left was treated with blocking peptide.)

Rabbit FBXO27 Polyclonal Antibody | anti-FBXO27 antibody

FBXO27 Antibody

Gene Names
FBXO27; FBG5; Fbx27
Reactivity
Human, Mouse, Rat
Predicted: Rabbit, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
FBXO27; Polyclonal Antibody; FBXO27 Antibody; F box G domain protein 5; F box only protein 27; F box protein 27; F box protein FBG5; FBG5; Fbx27; anti-FBXO27 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
FBXO27 Antibody detects endogenous levels of FBXO27.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
283
Applicable Applications for anti-FBXO27 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human FBXO27.
Tissue Specificity
Predominantly expressed in brain, heart and kidney. Expressed at lower levels in liver and lung.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from PANC-1 CELLS, using FBXO27 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from PANC-1 CELLS, using FBXO27 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-FBXO27 antibody
Description: Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex-type oligosaccharides.

Subunit Structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with SKP1 and CUL1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 32 kDa
Predicted: 32 kDa
NCBI Official Full Name
FBXO27 protein
NCBI Official Synonym Full Names
F-box protein 27
NCBI Official Symbol
FBXO27
NCBI Official Synonym Symbols
FBG5; Fbx27
NCBI Protein Information
F-box only protein 27
UniProt Protein Name
F-box only protein 27
Protein Family
UniProt Gene Name
FBXO27
UniProt Synonym Gene Names
FBG5; FBX27
UniProt Entry Name
FBX27_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXO27, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBXO27: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. Able to recognize and bind denatured glycoproteins, which are modified with complex- type oligosaccharides.

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: SCF ubiquitin ligase complex

Molecular Function: protein binding; glycoprotein binding

Research Articles on FBXO27

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Product Notes

The FBXO27 fbxo27 (Catalog #AAA9611758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXO27 Antibody reacts with Human, Mouse, Rat Predicted: Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's FBXO27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the FBXO27 fbxo27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXO27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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