Rabbit anti-Human, Mouse FBXL5 Polyclonal Antibody | anti-FBXL5 antibody

FBXL5, NT (FBXL5, FBL4, FBL5, FLR1, F-box/LRR-repeat protein 5, F-box and leucine-rich repeat protein 5, F-box protein FBL4/FBL5, p45SKP2-like protein)

Cat. Num. AAA645937

• Gene Names: FBXL5; FBL4; FBL5; FLR1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FBXL5; Polyclonal Antibody; NT (FBXL5; FBL4; FBL5; FLR1; F-box/LRR-repeat protein 5; F-box and leucine-rich repeat protein 5; F-box protein FBL4/FBL5; p45SKP2-like protein); Anti -FBXL5; anti-FBXL5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-FBXL5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the N-terminal region of human FBXL5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FBXL5 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab;)

Western Blot (WB)

(Western blot analysis of FBXL5 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(FBXL5 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FBXL5 Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FBXL5 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 302129689
• NCBI GeneID: 26234
• NCBI Accession #: NP_001180464.1
• NCBI GenBank Nucleotide #: NM_001193535.1
• UniProt Accession #: Q9UKA1; Q4W5A8; Q8NHP3; Q9NXN2; Q9P0I0; Q9P0X5; Q9UJT7; Q9UKC8; A8MSK4; B4DIB5
• Molecular Weight: 78,555 Da
• NCBI Official Full Name: F-box/LRR-repeat protein 5 isoform 4
• NCBI Official Synonym Full Names: F-box and leucine-rich repeat protein 5
• NCBI Official Symbol: FBXL5
• NCBI Official Synonym Symbols: FBL4; FBL5; FLR1
• NCBI Protein Information: F-box/LRR-repeat protein 5; F-box protein FBL5; p45SKP2-like protein; F-box protein FBL4/FBL5
• UniProt Protein Name: F-box/LRR-repeat protein 5
• Protein Family: F-box/LRR-repeat protein
• UniProt Gene Name: FBXL5
• UniProt Synonym Gene Names: FBL4; FBL5; FLR1
• UniProt Entry Name: FBXL5_HUMAN

NCBI Description

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains several tandem leucine-rich repeats. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2010]

Uniprot Description

Function: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued. Ref.11 Ref.12 Ref.13

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Interacts with ACO1/IRP1, IREB2/IRP2; the interaction depends on the 4Fe-4S cluster. Interacts with DCTN1/p150-glued. Ref.11 Ref.12 Ref.13

Subcellular location: Cytoplasm › perinuclear region Ref.11.

Domain: The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded. Ref.12 Ref.13

Post-translational modification: Polybiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome. Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.

Sequence similarities: Contains 1 F-box domain.Contains 7 LRR (leucine-rich) repeats.

Sequence caution: The sequence AAF66616.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF67489.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH30656.1 differs from that shown. Reason: Erroneous initiation. The sequence EAW92737.1 differs from that shown. Reason: Erroneous gene model prediction.