Western Blot (WB)
((2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat FAZF/TZFP/ZBTB32 Polyclonal Antibody | anti-ZBTB32 antibody
Goat anti-FAZF/TZFP/ZBTB32 Antibody
Gene Names
ZBTB32; Rog; FAXF; FAZF; TZFP; ZNF538
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FAZF/TZFP/ZBTB32; Polyclonal Antibody; Goat anti-FAZF/TZFP/ZBTB32 Antibody; ZBTB32; TZFP; FAZF; testis zinc finger protein; FAXF; zinc finger and BTB domain containing 32; Rog; ZNF538; OTTHUMP00000045790; repressor of GATA; ZBTB32 antibody; TZFP antibody; FAZF antibody; testis zinc finger protein antibody; FAXF antibody; zinc finger and BTB domain containing 32 antibody; Rog antibody; ZNF538 antibody; OTTHUMP00000045790 antibody; repressor of GATA antibody; FAZF / TZFP / ZBTB32; anti-ZBTB32 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RPSTSPCCPSSSTT
Sequence Length
487
Applicable Applications for anti-ZBTB32 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: A faint approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 53. 0kDa according to NP_055198). Recommended concentration: 2-5ug/ml.
Western Blot: A faint approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 53. 0kDa according to NP_055198). Recommended concentration: 2-5ug/ml.
Immunogen
Peptide with sequence RPSTSPCCPSSSTT, from the C Terminus of the protein sequence according to NP_055198.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,963 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 32 isoform 1
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 32
NCBI Official Symbol
ZBTB32
NCBI Official Synonym Symbols
Rog; FAXF; FAZF; TZFP; ZNF538
NCBI Protein Information
zinc finger and BTB domain-containing protein 32
UniProt Protein Name
Zinc finger and BTB domain-containing protein 32
Protein Family
UniProt Gene Name
ZBTB32
UniProt Synonym Gene Names
FAZF; TZFP; ZNF538
UniProt Entry Name
ZBT32_HUMAN
Uniprot Description
ZBTB32: DNA-binding protein that binds to the to a 5'- TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; protein binding; transcription corepressor activity; zinc ion binding
Biological Process: DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on ZBTB32
Similar Products
Product Notes
The ZBTB32 zbtb32 (Catalog #AAA420783) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FAZF/TZFP/ZBTB32 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAZF/TZFP/ZBTB32 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: A faint approx 55kDa band observed in Human Bone Marrow lysates (calculated MW of 53. 0kDa according to NP_055198). Recommended concentration: 2-5ug/ml. Researchers should empirically determine the suitability of the ZBTB32 zbtb32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RPSTSPCCPS SSTT. It is sometimes possible for the material contained within the vial of "FAZF/TZFP/ZBTB32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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