Rabbit anti-Human FAS Polyclonal Antibody | anti-FAS antibody

FAS ligand Antibody

Cat. Num. AAA9411886

• Gene Names: FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: FAS; Polyclonal Antibody; FAS ligand Antibody; Tumor necrosis factor ligand superfamily member 6; Fas antigen ligand; Fas ligand; CD178 antigen; CD95L protein; anti-FAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total FAS ligand protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 281

• Applicable Applications for anti-FAS antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human FAS ligand.
• Target Name: FAS ligand
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAS ligand antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cellls, using FAS ligand antibody.)

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells (Lane 2), using FAS ligand antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-FAS antibody

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.Alderson M.; J. Exp. Med. 181:71-77(1995).Takahashi T., Int. Immunol. 6:1567-1574(1994).Gregory S.G., ure 441:315-321(2006).

Product Categories/Family for anti-FAS antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557329
• NCBI GeneID: 356
• NCBI Accession #: NP_000630.1
• NCBI GenBank Nucleotide #: NM_000639.2
• UniProt Accession #: P48023; Q9BZP9
• Molecular Weight: 14,006 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: FASLG
• NCBI Official Synonym Symbols: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• Protein Family: Fascin
• UniProt Gene Name: FASLG
• UniProt Synonym Gene Names: APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_HUMAN

NCBI Description

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: extracellular space; lysosomal lumen; integral to plasma membrane; perinuclear region of cytoplasm; extracellular region; plasma membrane; caveola; nucleus; external side of plasma membrane

Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; cell-cell signaling; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of neuron apoptosis; positive regulation of cell proliferation; retinal cell programmed cell death; immune response

Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome