Rabbit Fas Polyclonal Antibody | anti-CD95; APO-1; TNFRSF 6 antibody

Fas polyclonal antibody

Cat. Num. AAA565230

• Gene Names: Fas; lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: Fas; Polyclonal Antibody; Fas polyclonal antibody; anti-CD95; APO-1; TNFRSF 6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse and rat Fas. Detects a band of ~45kDa by Western blot.
• Purity/Purification: Peptide Affinity Purified
• Form/Format: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
• Sequence Length: 327

• Applicable Applications for anti-CD95; APO-1; TNFRSF 6 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Immunohistochemistry (1:50); Western Blot (1:1,000, ECL); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~45kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to a portion of mouse Fas.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemical staining of human hepatoma tissue with Fas pAb.)

Related Product Information for anti-CD95; APO-1; TNFRSF 6 antibody

Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Both Fas and TNFR contain a 70 amino acid cytoplasmic "death domain" responsible for transmitting signals for apoptosis. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells.

Product Categories/Family for anti-CD95; APO-1; TNFRSF 6 antibody

Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 342187056
• NCBI GeneID: 14102
• UniProt Accession #: P25446; Q6GT31; Q9DCQ1
• Molecular Weight: 37,437 Da
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 6
• NCBI Official Synonym Full Names: Fas (TNF receptor superfamily member 6)
• NCBI Official Symbol: Fas
• NCBI Official Synonym Symbols: lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Fascin
• UniProt Gene Name: Fas
• UniProt Synonym Gene Names: Apt1; Tnfrsf6
• UniProt Entry Name: TNR6_MOUSE

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface; Receptor, cytokine; Apoptosis

Cellular Component: apical plasma membrane; CD95 death-inducing signaling complex; cell soma; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; lipid raft; membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; sarcolemma; secretory granule

Molecular Function: identical protein binding; kinase binding; protease binding; protein binding; protein complex binding; transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: activated T cell apoptosis; apoptosis; B cell mediated immunity; brain development; dendrite regeneration; gene expression; immune response; immunoglobulin production; induction of apoptosis via death domain receptors; inflammatory cell apoptosis; inflammatory response; negative regulation of apoptosis; negative regulation of B cell activation; negative thymic T cell selection; neuron apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of MAPKKK cascade; positive regulation of protein homooligomerization; protein homooligomerization; regulation of apoptosis; regulation of caspase activity; regulation of cell proliferation; regulation of gene expression; regulation of lymphocyte differentiation; regulation of myeloid cell differentiation; renal system process; response to glucocorticoid stimulus; response to lipopolysaccharide; response to toxin; signal transduction; spleen development; T cell homeostasis; transformed cell apoptosis; tumor necrosis factor-mediated signaling pathway