Rabbit anti-Human FAS Polyclonal Antibody | anti-FAS antibody

FAS, ID (Tumor Necrosis Factor Receptor Superfamily Member 6, FASLG Receptor, Apoptosis-mediating Surface Antigen FAS, Apo-1 Antigen, CD95, FAS, APT1, FAS1, TNFRSF6) (FITC)

Cat. Num. AAA6475599

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FAS; Polyclonal Antibody; ID (Tumor Necrosis Factor Receptor Superfamily Member 6; FASLG Receptor; Apoptosis-mediating Surface Antigen FAS; Apo-1 Antigen; CD95; APT1; FAS1; TNFRSF6) (FITC); anti-FAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FAS.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-FAS antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human FAS (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of FAS Antibody (Center) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FAS Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FAS antibody

Antibodies; Apoptosis

References

1. Feig, C., et.al., EMBO J. 26(1), 221-231 (2007). 2. Jenkins, M., et.al., J. Biol. Chem. 275(11), 7988-7993 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4507583
• NCBI GeneID: 355
• NCBI Accession #: NP_000034.1
• NCBI GenBank Nucleotide #: NM_000043.4
• UniProt Accession #: P25445; Q14292; Q14293; Q14294; Q14295; Q16652; Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9; A9UJX4; B6VNV4
• Molecular Weight: 37,732 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; TNF receptor superfamily member 6; apoptosis-mediating surface antigen FAS; Fas
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Fascin
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6
• UniProt Entry Name: TNR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine; Cell surface; Apoptosis

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: cell surface; cytoplasm; integral to membrane; plasma membrane; CD95 death-inducing signaling complex; nucleus; cytosol; lipid raft; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; signal transducer activity; transmembrane receptor activity; receptor activity; kinase binding

Biological Process: spleen development; caspase activation; circadian rhythm; regulation of myeloid cell differentiation; renal system process; transformed cell apoptosis; positive regulation of protein homooligomerization; positive regulation of apoptosis; apoptosis; regulation of lymphocyte differentiation; response to toxin; response to glucocorticoid stimulus; negative regulation of B cell activation; signal transduction; negative thymic T cell selection; regulation of apoptosis; inflammatory cell apoptosis; B cell mediated immunity; induction of apoptosis via death domain receptors; protein complex assembly; gene expression; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; negative regulation of apoptosis

Disease: Autoimmune Lymphoproliferative Syndrome