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Western Blot (WB) (Western Blot (WB) analysis of FARSLB polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Rabbit FARSLB Polyclonal Antibody | anti-FARSLB antibody

FARSLB Polyclonal Antibody

Gene Names
FARSB; FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
FARSLB; Polyclonal Antibody; FARSLB Polyclonal Antibody; Phenylalanine--tRNA ligase beta subunit; Phenylalanyl-tRNA synthetase beta subunit; PheRS; FARSB; FRSB; HSPC173; anti-FARSLB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
FARSLB polyclonal antibody detects endogenous levels of FARSLB protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
589
Applicable Applications for anti-FARSLB antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human FARSLB.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FARSLB polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)

Western Blot (WB) (Western Blot (WB) analysis of FARSLB polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Raw264. 7 cell lysateLane3:PC12 cell lysate)
Related Product Information for anti-FARSLB antibody
FARSLB (Phenylalanine--tRNA ligase beta chain), also known as FARSB, FRSB, PheHB or PheRS, is a 589 amino acid cytoplasmic protein that is the regulatory beta subunit of phenylalanine-tRNA synthetase. A highly conserved member of the aminoacyl-tRNA synthetase class IIc subfamily, FARSLB functions to enzymatically attach L-phenylalanine residues to terminal adenosines on tRNA(Phe). This is an ATP-dependent reaction that yields AMP, a diphosphate and an L-phenylalanine-tagged tRNA(Phe). FARSLB is expressed in brain, heart, kidney, pancreas, placenta and skeletal muscle and its expression is increased in malignant cell lines, suggesting a possible role for FARSLB in carcinogenesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 70kDa
NCBI Official Full Name
Phenylalanine--tRNA ligase beta subunit
NCBI Official Synonym Full Names
phenylalanyl-tRNA synthetase subunit beta
NCBI Official Symbol
FARSB
NCBI Official Synonym Symbols
FRSB; PheHB; PheRS; FARSLB; RILDBC; HSPC173; NEDBLLA
NCBI Protein Information
phenylalanine--tRNA ligase beta subunit
UniProt Protein Name
Phenylalanine--tRNA ligase beta subunit
UniProt Gene Name
FARSB
UniProt Synonym Gene Names
FARSLB; FRSB; PheRS
UniProt Entry Name
SYFB_HUMAN

NCBI Description

This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

FARSLB: a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq, Jul 2008]

Protein type: Ligase; EC 6.1.1.20; Translation

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: RNA binding; phenylalanine-tRNA ligase activity; magnesium ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; phenylalanyl-tRNA aminoacylation; gene expression

Research Articles on FARSLB

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Product Notes

The FARSLB farsb (Catalog #AAA3006740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FARSLB Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FARSLB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the FARSLB farsb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FARSLB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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