Rabbit anti-Human FARP1 Polyclonal Antibody | anti-FARP1 antibody

FARP1, NT (FARP1, CDEP, PLEKHC2, FERM, RhoGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein, Pleckstrin homology domain-containing family C member 2) (APC)

Cat. Num. AAA6298044

• Gene Names: FARP1; CDEP; PLEKHC2; PPP1R75; FARP1-IT1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FARP1; Polyclonal Antibody; NT (FARP1; CDEP; PLEKHC2; FERM; RhoGEF and pleckstrin domain-containing protein 1; Chondrocyte-derived ezrin-like protein; Pleckstrin homology domain-containing family C member 2) (APC); Pleckstrin homology domain-containing family C member 2; anti-FARP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-FARP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human FARP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(FARP1 Antibody (N-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the FARP1 antibody detected the FARP1 protein (arrow).)

Related Product Information for anti-FARP1 antibody

This gene was originally isolated through subtractive hybridization due to its increased expression in differentiated chondrocytes versus dedifferentiated chondrocytes. The resulting protein contains a predicted ezrin-like domain, a Dbl homology domain, and a pleckstrin homology domain. It is believed to be a member of the band 4.1 superfamily whose members link the cytoskeleton to the cell membrane. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-FARP1 antibody

Antibodies; Pleckstrin Homology Proteins

References

Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Evangelou, E., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 220-228 (2010): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5031633
• NCBI GeneID: 10160
• NCBI Accession #: NP_005757.1; NP_001001715.2
• NCBI GenBank Nucleotide #: NM_005766.3
• UniProt Accession #: Q9Y4F1; Q5JVI9; Q6IQ29
• Molecular Weight: 14,138 Da
• NCBI Official Full Name: FERM, RhoGEF and pleckstrin domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: FERM, ARH/RhoGEF and pleckstrin domain protein 1
• NCBI Official Symbol: FARP1
• NCBI Official Synonym Symbols: CDEP; PLEKHC2; PPP1R75; FARP1-IT1
• NCBI Protein Information: FERM, RhoGEF and pleckstrin domain-containing protein 1
• UniProt Protein Name: FERM, RhoGEF and pleckstrin domain-containing protein 1
• Protein Family: FERM, RhoGEF and pleckstrin domain-containing protein
• UniProt Gene Name: FARP1
• UniProt Synonym Gene Names: CDEP; PLEKHC2
• UniProt Entry Name: FARP1_HUMAN

NCBI Description

This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]