Rabbit anti-Human PML Polyclonal Antibody | anti-PML antibody

PML, Sumoylation Site (Probable Transcription Factor PML, Tripartite Motif-containing Protein 19, RING Finger Protein 71, MYL, RNF71, TRIM19) (PE)

Cat. Num. AAA6494053

• Gene Names: PML; MYL; RNF71; PP8675; TRIM19
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: PML; Polyclonal Antibody; Sumoylation Site (Probable Transcription Factor PML; Tripartite Motif-containing Protein 19; RING Finger Protein 71; MYL; RNF71; TRIM19) (PE); anti-PML antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the Sumoylation Site of human PML.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 829

• Applicable Applications for anti-PML antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 470-499 at the Sumoylation Site of human PML, UniProt Accession #P29590.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS626500. PML (arrow) was detected usingMBS626500.)

Immunohistochemistry (IHC)

(Immunohistochemistry in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS626500, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS626500 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with Hela cells using MBS626500 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS626500 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PML antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Puccetti, E., et al., Oncogene 22(44):6900-6908 (2003). 2. Favre, M., et al., J. Acquir. Immune Defic. Syndr. 34(2):127-133 (2003). 3. Kawai, T., et al., Mol. Cell. Biol. 23(17):6174-6186 (2003). 4. Louria-Hayon, I., et al., J. Biol. Chem. 278(35):33134-33141 (2003). 5. Moller, A., et al., Cancer Res. 63(15):4310-4314 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 15451765
• NCBI GeneID: 5371
• NCBI Accession #: NP_150242
• NCBI GenBank Nucleotide #: NP_150242.1
• UniProt Accession #: P29590
• Molecular Weight: 97.516
• NCBI Official Full Name: protein PML isoform 9
• NCBI Official Synonym Full Names: PML nuclear body scaffold
• NCBI Official Symbol: PML
• NCBI Official Synonym Symbols: MYL; RNF71; PP8675; TRIM19
• NCBI Protein Information: protein PML
• UniProt Protein Name: Protein PML
• Protein Family: Protein
• UniProt Gene Name: PML
• UniProt Synonym Gene Names: MYL; PP8675; RNF71; TRIM19
• UniProt Entry Name: PML_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PML: a zinc-finger protein that can regulate transcription and can localize to nuclear bodies. Cytoplasmic forms regulate glycolysis by inhibiting the tetrameric form of PKM2. Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Sumoylated forms interact with SATB1 and localize to the PML nuclear bodies. Sumoylation on three sites is required for nuclear body formation. Sumoylation on Lys-160 is a prerequisite for sumoylation on Lys-65. The B1 box and the RING finger are also required for localization to nuclear bodies. May play an important role in recruitment of ELF4 into PML nuclear bodies. A chromosomal aberration involving PML can cause acute promyelocytic leukemia (APL). Seven alternatively-spliced human isoforms have been reported.

Protein type: Ubiquitin conjugating system; Tumor suppressor; Transcription factor; Nucleolus; Oncoprotein

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; PML body; nuclear membrane; nuclear matrix; early endosome membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; SUMO binding; DNA binding; zinc ion binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription coactivator activity; SMAD binding; cobalt ion binding

Biological Process: retinoic acid receptor signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; viral reproduction; apoptosis; positive regulation of apoptosis involved in mammary gland involution; regulation of MHC class I biosynthetic process; PML body organization and biogenesis; SMAD protein nuclear translocation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; entrainment of circadian clock by photoperiod; negative regulation of cell proliferation; positive regulation of histone deacetylation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; response to gamma radiation; protein complex assembly; negative regulation of mitotic cell cycle; circadian regulation of gene expression; maintenance of protein localization in nucleus; cell cycle arrest; defense response to virus; negative regulation of telomere maintenance via telomerase; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to UV; caspase activation; regulation of protein amino acid phosphorylation; cell fate commitment; protein stabilization; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; common-partner SMAD protein phosphorylation; regulation of circadian rhythm; negative regulation of telomerase activity; endoplasmic reticulum calcium ion homeostasis; negative regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; response to hypoxia; innate immune response; myeloid cell differentiation; negative regulation of translation in response to oxidative stress; negative regulation of cell growth; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; induction of apoptosis by oxidative stress; protein targeting