Western Blot (WB)
(Western Blot: Sample: Recombinant FXR, Rat)
Rabbit anti-Rat Farnesoid X Receptor Polyclonal Antibody | anti-FXR antibody
Farnesoid X Receptor (FXR) Polyclonal Antibody
Gene Names
Fxr1; Fxr1h
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Farnesoid X Receptor; Polyclonal Antibody; Farnesoid X Receptor (FXR) Polyclonal Antibody; NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1; Group H; Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14; anti-FXR antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against FXR. It has been selected for its ability to recognize FXR in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-FXR antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Rat
Organism Species
Rat
Fragment
FXR (Leu375~Asp568)
Quality Control
Content: The quality control contains recombinant FXR (Leu375~Asp568) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062434
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant FXR, Rat)
Western Blot (WB)
(Western Blot: Sample: Recombinant FXR, Rat)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,947 Da
NCBI Official Full Name
fragile X mental retardation syndrome-related protein 1
NCBI Official Synonym Full Names
FMR1 autosomal homolog 1
NCBI Official Symbol
Fxr1
NCBI Official Synonym Symbols
Fxr1h
NCBI Protein Information
fragile X mental retardation syndrome-related protein 1
UniProt Protein Name
Fragile X mental retardation syndrome-related protein 1
UniProt Gene Name
Fxr1
Uniprot Description
FXR1: RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs. Interacts with FMR1 and FXR2. Interacts with TDRD3, and with CYFIP2 but not with CYFIP1. Expressed in all tissues examined including heart, brain, kidney and testis. Belongs to the FMR1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 2q24
Cellular Component: axon; costamere; cytoplasm; cytosol; dendrite; dendritic spine; membrane; nucleus; perinuclear region of cytoplasm; polysome
Molecular Function: mRNA 3'-UTR binding; mRNA binding; protein heterodimerization activity; protein homodimerization activity; RNA binding; RNA strand annealing activity
Biological Process: cell differentiation; muscle organ development; negative regulation of translation
Similar Products
Product Notes
The FXR fxr1 (Catalog #AAA2032490) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Farnesoid X Receptor (FXR) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Farnesoid X Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the FXR fxr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Farnesoid X Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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