Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, 293T whole cell lysateAll lanes: FAM83D antibody at 4.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65, 63 KDaObserved band size: 65 KDa)
Rabbit anti-Human FAM83D Polyclonal Antibody | anti-FAM83D antibody
FAM83D Antibody
Gene Names
FAM83D; CHICA; C20orf129; dJ616B8.3
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
FAM83D; Polyclonal Antibody; FAM83D Antibody; Protein FAM83D; Spindle protein CHICA1; anti-FAM83D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
585
Applicable Applications for anti-FAM83D antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Protein FAM83D protein (339-470AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, 293T whole cell lysateAll lanes: FAM83D antibody at 4.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65, 63 KDaObserved band size: 65 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, 293T whole cell lysateAll lanes: FAM83D antibody at 4.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 65, 63 KDaObserved band size: 65 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7110523 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with MBS7110523 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-FAM83D antibody
Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.
Product Categories/Family for anti-FAM83D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,596 Da
NCBI Official Full Name
protein FAM83D
NCBI Official Synonym Full Names
family with sequence similarity 83 member D
NCBI Official Symbol
FAM83D
NCBI Official Synonym Symbols
CHICA; C20orf129; dJ616B8.3
NCBI Protein Information
protein FAM83D
UniProt Protein Name
Protein FAM83D
Protein Family
UniProt Gene Name
FAM83D
Uniprot Description
Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed:24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed:25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.
Research Articles on FAM83D
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Product Notes
The FAM83D fam83d (Catalog #AAA7110523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAM83D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FAM83D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the FAM83D fam83d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAM83D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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