Rabbit anti-Mouse, Rat NR3B Polyclonal Antibody | anti-nodal antibody

NR3B

Cat. Num. AAA611857

• Gene Names: nodal; nr4; Xnr4; Xnr-4; nr3-A; nr3-B; nodal4; Xtnr3-A; xtnr3-b; nodal3-A; nodal3-B
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NR3B; Polyclonal Antibody; Anti -NR3B; anti-nodal antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NR3B, Mr ~98kD. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-nodal antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot : 0.5-2ug/ml detected NR3B in a rat brain microsomal protein preparation.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 916-930 (C-RRVRRAVVERERRVR) of mouse NR3B, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 14/15 identical amino acids in rat.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: Rat brain microsomal protein preparation was probed with MBS611857 (2ug/ml).)

Product Categories/Family for anti-nodal antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 45360939
• NCBI GeneID: 394458
• NCBI Accession #: NP_988864.1
• NCBI GenBank Nucleotide #: NM_203533.1
• UniProt Accession #: Q800B9; Q800C0
• Molecular Weight: 45,849 Da
• NCBI Official Full Name: nodal homolog 3-A
• NCBI Official Synonym Full Names: nodal homolog
• NCBI Official Symbol: nodal
• NCBI Official Synonym Symbols: nr4; Xnr4; Xnr-4; nr3-A; nr3-B; nodal4; Xtnr3-A; xtnr3-b; nodal3-A; nodal3-B
• NCBI Protein Information: nodal homolog 3-A; Nodal homolog 4; nodal-related protein 3-A
• UniProt Protein Name: Nodal homolog 3-B
• Protein Family: Nodal
• UniProt Gene Name: nodal3-B
• UniProt Synonym Gene Names: nr3-B
• UniProt Entry Name: NOD3B_XENTR

Uniprot Description

Function: Exhibits mesoderm-dorsalizing activity and neural-inducing activity, but lacks mesoderm-inducing activity. Regulates the expression of specific mesodermal and neural genes. Induces convergent extension movements at the embryonic midline by activating the fgf signaling pathway to induce t/bra expression in the organizer region. Acts with wnt11 to induce Spemann organizer cells and induce axis formation

By similarity. The unprocessed protein antagonizes bmp-signaling. Ref.1

Subunit structure: Monomer

By similarity. The propeptide region interacts with bmp4 in a non-covalent manner. Ref.1

Subcellular location: Secreted

By similarity.

Tissue specificity: Expressed in the dorsal marginal region of late blastula, becoming restricted to the Spemann organizer at the early gastrula stage. Ref.1

Developmental stage: First detected at the blastula stage (stage 8). Expression peaks through the mid-blastula (stage 8.5) to early gastrula (stage 10) and then decreases during gastrulation. Ref.1

Domain: The propeptide region is both necessary and sufficient for bmp-inhibitory activity. The propeptide region and the N- and C-terminal thirds of the mature protein are necessary for neural induction activity. Although cleavage doesn't appear essential for activity, residues surrounding the cleavage site are necessary for activity

By similarity.

Sequence similarities: Belongs to the TGF-beta family.