Western Blot (WB)
(Figure 1. Western blot analysis of FADD using anti-FADD antibody (MBS1753296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse brain tissue lysatesLane 2: mouse stomach tissue lysatesLane 3: mouse lung tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse thymus tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # MBS1753296) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD. )
Rabbit anti-Mouse, Rat FADD Polyclonal Antibody | anti-Fadd antibody
Anti-FADD Antibody
IHC-P: 2-5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|
Western Blot (WB)
(Figure 1. Western blot analysis of FADD using anti-FADD antibody (MBS1753296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse brain tissue lysatesLane 2: mouse stomach tissue lysatesLane 3: mouse lung tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse thymus tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # MBS1753296) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of FADD using anti-FADD antibody (MBS1753296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: mouse brain tissue lysatesLane 2: mouse stomach tissue lysatesLane 3: mouse lung tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse thymus tissue lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # MBS1753296) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD. )
Western Blot (WB)
(Figure 2. Western blot analysis of FADD using anti-FADD antibody (MBS1753296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: rat stomach tissue lysatesLane 3: rat lung tissue lysatesLane 4: rat liver tissue lysatesLane 5: rat kidney tissue lysatesLane 6: rat spleen tissue lysatesLane 7: rat thymus tissue lysatesLane 8: rat PC-12 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # MBS1753296) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD. )
Western Blot (WB)
(Figure 2. Western blot analysis of FADD using anti-FADD antibody (MBS1753296).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat brain tissue lysatesLane 2: rat stomach tissue lysatesLane 3: rat lung tissue lysatesLane 4: rat liver tissue lysatesLane 5: rat kidney tissue lysatesLane 6: rat spleen tissue lysatesLane 7: rat thymus tissue lysatesLane 8: rat PC-12 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # MBS1753296) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for FADD at approximately 30KD. The expected band size for FADD is at 23KD. )
2. Kim, P. K. M., Dutra, A. S., Chandrasekharappa, S. C., Puck, J. M. Genomic structure and mapping of human FADD, an intracellular mediator of lymphocyte apoptosis. J. Immun. 157: 5461-5466, 1996.
3. Lee, H. -J., Pyo, J. -O., Oh, Y., Kim, H. -J., Hong, S., Jeon, Y. -J., Kim, H., Cho, D. -H., Woo, H. -N., Song, S., Nam, J. -H., Kim, H. J., Kim, K. -S., Jung, Y. -K. AK2 activates a novel apoptotic pathway through formation of a complex with FADD and caspase-10. Nature Cell Biol. 9: 1303-1310, 2007.
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Product Notes
The Fadd fadd (Catalog #AAA1753296) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FADD Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FADD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM). WB: 0.1-0.25ug/ml|Mouse, Rat| IHC-P: 2-5ug/ml|Mouse| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|. Researchers should empirically determine the suitability of the Fadd fadd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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