Rabbit anti-Human, Mouse FABP3 Polyclonal Antibody | anti-FABP3 antibody

FABP3, NT (Fatty Acid-binding Protein, Heart, Heart-type Fatty Acid-binding Protein, H-FABP, Fatty Acid-binding Protein 3, Muscle Fatty Acid-binding Protein, M-FABP, Mammary-derived Growth Inhibitor, MDGI, FABP11) (HRP)

Cat. Num. AAA6475657

• Gene Names: FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: FABP3; Polyclonal Antibody; NT (Fatty Acid-binding Protein; Heart; Heart-type Fatty Acid-binding Protein; H-FABP; Fatty Acid-binding Protein 3; Muscle Fatty Acid-binding Protein; M-FABP; Mammary-derived Growth Inhibitor; MDGI; FABP11) (HRP); anti-FABP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FABP3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FABP3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human FABP3 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of FABP3 antibody (N-term) in mouse heart tissue lysates (35ug/lane). FABP3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with FABP3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using FABP3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of FABP3 (arrow) using rabbit polyclonal FABP3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FABP3 gene.)

Product Categories/Family for anti-FABP3 antibody

Antibodies; Fatty Acid Binding Protein

References

1. Iwayama, Y., Am. J. Med. Genet. B Neuropsychiatr. Genet. (2009). 2. Lazary, A., Eur. J. Endocrinol. 159(2), 187-196 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758328
• NCBI GeneID: 2170
• NCBI Accession #: NP_004093
• NCBI GenBank Nucleotide #: NM_004102.4
• UniProt Accession #: P05413; Q5VV93; Q99957; B2RAB6
• Molecular Weight: 19.1 kDa (170aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: fatty acid-binding protein, heart isoform 2
• NCBI Official Synonym Full Names: fatty acid binding protein 3
• NCBI Official Symbol: FABP3
• NCBI Official Synonym Symbols: MDGI; FABP11; H-FABP; M-FABP; O-FABP
• NCBI Protein Information: fatty acid-binding protein, heart
• UniProt Protein Name: Fatty acid-binding protein, heart
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP3
• UniProt Synonym Gene Names: FABP11; MDGI; H-FABP; MDGI; M-FABP
• UniProt Entry Name: FABPH_HUMAN

NCBI Description

The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

FABP3: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: extracellular space; sarcoplasm; cytosol

Molecular Function: protein binding; icosatetraenoic acid binding; cytoskeletal protein binding; long-chain fatty acid transporter activity

Biological Process: response to drug; negative regulation of cell proliferation; cholesterol homeostasis; regulation of fatty acid oxidation; fatty acid metabolic process; response to insulin stimulus; phospholipid homeostasis