Monkey Activating Transcription Factor 1 ELISA Kit | ATF1 elisa kit

Monkey Activating Transcription Factor 1 ELISA Kit

Cat. Num. AAA746888

• Gene Names: ATF1; TREB36; EWS-ATF1; FUS/ATF-1
• Reactivity: Monkey
• Synonyms: Activating Transcription Factor 1; Monkey Activating Transcription Factor 1 ELISA Kit; ATF1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ATF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ATF1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATF1 elisa kit

Principle of the Assay: ATF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF1 antibody and an ATF1-HRP conjugate. The assay sample and buffer are incubated together with ATF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF1 concentration since ATF1 from samples and ATF1-HRP conjugate compete for the anti-ATF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF1 from the sample, fewer sites are left to bind ATF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATF1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 119578555
• NCBI GeneID: 466
• Molecular Weight: 15,295 Da
• NCBI Official Full Name: activating transcription factor 1
• NCBI Official Synonym Full Names: activating transcription factor 1
• NCBI Official Symbol: ATF1
• NCBI Official Synonym Symbols: TREB36; EWS-ATF1; FUS/ATF-1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-1
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF1
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-1
• UniProt Entry Name: ATF1_HUMAN

NCBI Description

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010]

Uniprot Description

ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; protein complex binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; response to organic cyclic substance; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; cellular protein complex assembly; response to cobalt ion; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication