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Immunohistochemistry (IHC) (MBS9610417 At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Rabbit FABP1 Polyclonal Antibody | anti-FABP1 antibody

Phospho-FABP1 (Ser11) Antibody

Gene Names
FABP1; FABPL; L-FABP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
FABP1; Polyclonal Antibody; Phospho-FABP1 (Ser11) Antibody; FABP 1; FABPL; FABPL_HUMAN; Fatty Acid Binding Protein 1; Fatty acid binding protein 1 liver; Fatty Acid Binding Protein; Fatty acid-binding protein 1; Fatty acid-binding protein; Fatty acid-binding protein liver; L FABP; L-FABP; liver; Liver-type fatty acid-binding protein; anti-FABP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-FABP1(Ser11) Antibody detects endogenous levels of FABP1 only when phosphorylated at Ser11.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
127
Applicable Applications for anti-FABP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human FABP1 around the phosphorylation site of Ser11.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9610417 At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC) (MBS9610417 At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 , using Phospho-FABP1(Ser11) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 , using Phospho-FABP1(Ser11) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Related Product Information for anti-FABP1 antibody
Description: Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).

Similarity: Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
14kDa
NCBI Official Full Name
L-FABP
NCBI Official Synonym Full Names
fatty acid binding protein 1
NCBI Official Symbol
FABP1
NCBI Official Synonym Symbols
FABPL; L-FABP
NCBI Protein Information
fatty acid-binding protein, liver
UniProt Protein Name
Fatty acid-binding protein, liver
UniProt Gene Name
FABP1
UniProt Synonym Gene Names
FABPL; L-FABP
UniProt Entry Name
FABPL_HUMAN

NCBI Description

This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]

Uniprot Description

Function: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.

Subcellular location: Cytoplasm.

Domain: Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

Sequence similarities: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Research Articles on FABP1

Similar Products

Product Notes

The FABP1 fabp1 (Catalog #AAA9610417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-FABP1 (Ser11) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FABP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the FABP1 fabp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FABP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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