Western Blot (WB)
(Western blot analysis of TFIIE-alpha expression in Jurkat (A), A431 (B), mouse brain (C), mouse spleen (D) whole cell lysates.)
Rabbit TFIIE-alpha Polyclonal Antibody | anti-TFIIE-alpha antibody
Anti-TFIIE-alpha Antibody
Gene Names
GTF2E1; FE; TF2E1; TFIIE-A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
TFIIE-alpha; Polyclonal Antibody; Anti-TFIIE-alpha Antibody; TF2E1; General transcription factor IIE subunit 1; General transcription factor IIE 56 kDa subunit; Transcription initiation factor IIE subunit alpha; anti-TFIIE-alpha antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of TFIIE-alpha protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-TFIIE-alpha antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
IF/ICC: 1:50-1:200
Immunogen
Recombinant fusion protein of human TFIIE-alpha. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of TFIIE-alpha expression in Jurkat (A), A431 (B), mouse brain (C), mouse spleen (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TFIIE-alpha expression in Jurkat (A), A431 (B), mouse brain (C), mouse spleen (D) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of TFIIE-alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of TFIIE-alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Related Product Information for anti-TFIIE-alpha antibody
Rabbit polyclonal antibody to TFIIE-alpha
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,452 Da
NCBI Official Full Name
Homo sapiens general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1), mRNA
NCBI Official Synonym Full Names
general transcription factor IIE, polypeptide 1, alpha 56kDa
NCBI Official Symbol
GTF2E1
NCBI Official Synonym Symbols
FE; TF2E1; TFIIE-A
NCBI Protein Information
general transcription factor IIE subunit 1; TFIIE-alpha; general transcription factor IIE 56 kDa subunit; transcription initiation factor IIE subunit alpha
UniProt Protein Name
General transcription factor IIE subunit 1
UniProt Gene Name
GTF2E1
UniProt Synonym Gene Names
TF2E1; TFIIE-alpha
UniProt Entry Name
T2EA_HUMAN
Research Articles on TFIIE-alpha
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Product Notes
The TFIIE-alpha gtf2e1 (Catalog #AAA8306838) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TFIIE-alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TFIIE-alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the TFIIE-alpha gtf2e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFIIE-alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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