Rabbit anti-Human, Mouse F162A Polyclonal Antibody | anti-F162A antibody

F162A, CT (FAM162A, C3orf28, E2IG5, Protein FAM162A, E2-induced gene 5 protein, Growth and transformation-dependent protein) (Azide free) (HRP)

Cat. Num. AAA6297376

• Gene Names: FAM162A; E2IG5; HGTD-P; C3orf28
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: F162A; Polyclonal Antibody; CT (FAM162A; C3orf28; E2IG5; Protein FAM162A; E2-induced gene 5 protein; Growth and transformation-dependent protein) (Azide free) (HRP); FAM162A; Growth and transformation-dependent protein; anti-F162A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-F162A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: F162A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from the C-terminal region of human F162A.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(F162A Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the F162A antibody detected the F162A protein (arrow).)

Product Categories/Family for anti-F162A antibody

Antibodies; KIAA; orf

References

O'Seaghdha, C.M., et al. Hum. Mol. Genet. 19(21):4296-4303(2010). Qu, Y., et al. Stroke 40(8):2843-2848(2009). Cho, Y.E., et al. Hum. Pathol. 40(7):975-981(2009). Kim, J.Y., et al. FEBS Lett. 580(13):3270-3275(2006). Lee, M.J., et al. Mol. Cell. Biol. 24(9):3918-3927(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 49355721
• NCBI GeneID: 26355
• NCBI Accession #: NP_055182.3
• NCBI GenBank Nucleotide #: NM_014367.3
• UniProt Accession #: Q96A26; Q9NRN6; Q9UJX8
• Molecular Weight: Predicted MW: 17kDa
• NCBI Official Full Name: protein FAM162A
• NCBI Official Synonym Full Names: family with sequence similarity 162 member A
• NCBI Official Symbol: FAM162A
• NCBI Official Synonym Symbols: E2IG5; HGTD-P; C3orf28
• NCBI Protein Information: protein FAM162A
• UniProt Protein Name: Protein FAM162A
• UniProt Gene Name: FAM162A
• UniProt Synonym Gene Names: C3orf28; E2IG5; HGTD-P

Uniprot Description

Proposed to be involved in regulation of apoptosis; the exact mechanism may differ between cell types/tissues. May be involved in hypoxia-induced cell death of transformed cells implicating cytochrome C release and caspase activation (such as CASP9) and inducing mitochondrial permeability transition. May be involved in hypoxia-induced cell death of neuronal cells probably by promoting release of AIFM1 from mitochondria to cytoplasm and its translocation to the nucleus; however, the involvement of caspases has been reported conflictingly.