Rabbit anti-Human F12 Polyclonal Antibody | anti-F12 antibody

F12, NT (F12, Coagulation factor XII, Hageman factor, Coagulation factor XIIa heavy chain, Beta-factor XIIa part 1, Beta-factor XIIa part 2, Coagulation factor XIIa light chain) (Azide free) (HRP)

Cat. Num. AAA6297343

• Gene Names: F12; HAF; HAE3; HAEX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: F12; Polyclonal Antibody; NT (F12; Coagulation factor XII; Hageman factor; Coagulation factor XIIa heavy chain; Beta-factor XIIa part 1; Beta-factor XIIa part 2; Coagulation factor XIIa light chain) (Azide free) (HRP); Coagulation factor XIIa light chain; anti-F12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-F12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: F12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human F12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(F12 Antibody (N-term) western blot analysis in K562(lane 1),CEM(lane 2),MDA-MB435(lane 3) cell line lysates (35ug/lane).This demonstrates the F12 antibody detected the F12 protein (arrow).)

Related Product Information for anti-F12 antibody

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI.

Product Categories/Family for anti-F12 antibody

Antibodies; Coagulation Factors

References

Houlihan, L.M., et al. Am. J. Hum. Genet. 86(4):626-631(2010). Calafell, F., et al. Hum. Mol. Genet. 19(3):517-525(2010). Katakami, N., et al. Diabetes Care 33(2):390-395(2010). Back, J., et al. Biochem. Biophys. Res. Commun. 391(1):11-17(2010). Joseph, K., et al. Ann. Allergy Asthma Immunol. 104(1):50-54(2010). Bunkenborg, J., et al. Proteomics 4(2):454-465(2004). Hiller, O., et al. J. Biol. Chem. 275(42):33008-33013(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 145275213
• NCBI GeneID: 2161
• NCBI Accession #: NP_000496.2
• NCBI GenBank Nucleotide #: NM_000505.3
• UniProt Accession #: P00748; P78339
• Molecular Weight: 67,792 Da
• NCBI Official Full Name: coagulation factor XII
• NCBI Official Synonym Full Names: coagulation factor XII (Hageman factor)
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: HAF; HAE3; HAEX
• NCBI Protein Information: coagulation factor XII; Hageman factor; beta-factor XIIa part 1; beta-factor XIIa part 2; coagulation factor XIIa heavy chain; coagulation factor XIIa light chain
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Synonym Gene Names: HAF
• UniProt Entry Name: FA12_HUMAN

NCBI Description

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Uniprot Description

F12: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta- factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Defects in F12 are the cause of factor XII deficiency (FA12D); also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3); also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g. during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. Belongs to the peptidase S1 family.

Protein type: Protease; Chaperone; Secreted, signal peptide; Secreted; EC 3.4.21.38

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity; misfolded protein binding

Biological Process: response to misfolded protein; positive regulation of blood coagulation; proteolysis; protein autoprocessing; fibrinolysis; plasma kallikrein-kinin cascade; innate immune response; positive regulation of fibrinolysis; zymogen activation; protein processing; blood coagulation; blood coagulation, intrinsic pathway; Factor XII activation

Disease: Angioedema, Hereditary, Type Iii; Factor Xii Deficiency