Rabbit anti-Human Exportin 5 Polyclonal Antibody | anti-XPO5 antibody

Polyclonal Antibody to Exportin 5 (XPO5)

Cat. Num. AAA2033729

• Gene Names: XPO5; exp5
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Exportin 5; Polyclonal Antibody; Polyclonal Antibody to Exportin 5 (XPO5); RANBP21; Exp5; Ran-binding protein 21; anti-XPO5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against XPO5. It has been selected for its ability to recognize XPO5 in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 1204

• Applicable Applications for anti-XPO5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-1:5000
• Fragment: XPO5 (Ala2~Gln180)
• Conjugate: No Conjugate
• Cross Reactivity: Human
• Immunoglobulin Type: IgG
• Quality Control: Content: The quality control contains recombinant XPO5 (Ala2~Gln180) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2075999
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded brain tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 22748937
• NCBI GeneID: 57510
• NCBI Accession #: NP_065801.1
• NCBI GenBank Nucleotide #: NM_020750.2
• UniProt Accession #: Q9HAV4; Q5JTE6; Q96G48; Q96HN3; Q9BWM6; Q9BZV5; Q9H9M4; Q9NT89; Q9NW39; Q9ULC9
• Molecular Weight: 136,311 Da
• NCBI Official Full Name: exportin-5
• NCBI Official Synonym Full Names: exportin 5
• NCBI Official Symbol: XPO5
• NCBI Official Synonym Symbols: exp5
• NCBI Protein Information: exportin-5
• UniProt Protein Name: Exportin-5
• Protein Family: Exportin
• UniProt Gene Name: XPO5
• UniProt Synonym Gene Names: KIAA1291; RANBP21; Exp5

NCBI Description

This gene encodes a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. The encoded protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process. [provided by RefSeq, Aug 2011]

Uniprot Description

Exportin-5: mediates the nuclear export of proteins bearing double-stranded RNA binding domains and double-stranded RNA. Overexpression enhances RNA interference mediated by shRNAs and miRNAs. In the nucleus it binds cooperatively to its cargo and to Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. Exp5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Karyopherin; Nuclear export; Nuclear import; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; cytosol; nuclear RNA export factor complex; nucleoplasm; nucleus

Molecular Function: mRNA binding; nuclear export signal receptor activity; protein binding; Ran GTPase binding; RNA binding; tRNA binding

Biological Process: pre-miRNA export from nucleus; protein export from nucleus; regulation of protein export from nucleus