Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using EVPL antibody.)
Rabbit EVPL Polyclonal Antibody | anti-EVPL antibody
EVPL Polyclonal Antibody
Gene Names
EVPL; EVPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
EVPL; Polyclonal Antibody; EVPL Polyclonal Antibody; EVPK; anti-EVPL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EVPL polyclonal antibody detects endogenous levels of EVPL protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
2055
Applicable Applications for anti-EVPL antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human EVPL
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using EVPL antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cell lines, using EVPL antibody.)
Related Product Information for anti-EVPL antibody
Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease that is associated with underlying neoplasms. PNP sera react with multiple plakin family proteins, among which only envoplakin and periplakin are constantly detected. Envoplakin, a membrane-associated precursor of the epidermal cornified envelope and desmosomes, is a member of the plakin family of proteins. Envoplakin is expressed in epidermal and esophageal keratinocytes and nonepithelial stratified squamous epithelia, but not in simple epithelia or nonepithelial cells. Envoplakin co-localizes with desmoplakin at desmosomes and on keratin filaments throughout the differentiated layers of the epidermis, but mainly accumulates in nuclear and cytoplasmic aggregates with associated intermediate filaments. The envoplakin rod domain is required for aggregation and the linker domain is required for intermediate filament association. The distribution of envoplakin at the interdesmosomal plasma membrane depends on heterodimerization with periplakin
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 270kDa
NCBI Official Full Name
envoplakin isoform 1
NCBI Official Synonym Full Names
envoplakin
NCBI Official Symbol
EVPL
NCBI Official Synonym Symbols
EVPK
NCBI Protein Information
envoplakin
UniProt Protein Name
Envoplakin
Protein Family
UniProt Gene Name
EVPL
NCBI Description
This gene encodes a member of the plakin family of proteins that forms a component of desmosomes and the epidermal cornified envelope. This gene is located in the tylosis oesophageal cancer locus on chromosome 17q25, and its deletion is associated with both familial and sporadic forms of oesophageal squamous cell carcinoma. Patients suffering from the autoimmune mucocutaneous disorder, paraneoplastic pemphigus, develop antibodies against the encoded protein. [provided by RefSeq, Jul 2016]
Uniprot Description
Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
Research Articles on EVPL
Similar Products
Product Notes
The EVPL evpl (Catalog #AAA3009572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EVPL Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EVPL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the EVPL evpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EVPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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