Dipeptidyl Peptidase 9 Recombinant Protein | DPP9 recombinant protein

Recombinant Dipeptidyl Peptidase 9 (DPP9)

Cat. Num. AAA2030303

• Gene Names: DPP9; DP9; DPLP9; DPRP2; DPRP-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Dipeptidyl Peptidase 9; Recombinant Dipeptidyl Peptidase 9 (DPP9); DPP9 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; VNEQTK LVYFQGTKDT PLEHHLYVVS YESAGEIVRL TTLGFSHSCSMSQSFDMFVS HYSSVSTPPC VHVYKLSGPD DDPLHKQPRF WASMMEAANCPPDYVPPEIF HFHTRADVQL YGMIYKPHTL QPGRKHPTVL FVYGGPQVQLVNNSFKGIKY LRLNTLASLG YAVVVIDGRG SCQRGLHFEG ALKNQMGQVEIEDQVEGLQY VAEKYGFIDL SRVAIHGWSY GGFLSLMGLI HKPQVFKVAIAGAPVTVWMA YDTGYTERYM DVPENNQQGY EAGSVALHVE KLPNEPNRLLILHGFLDENV HFFHTNFLVS QLIRAGKPYQ LQIYPNERHS IRCRESGEHYEVTLLHFLQE HL
• Sequence Length: 892

• Applicable Applications for DPP9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 70.7kDa
• Accurate Molecular Mass (KD): 71kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Val505~Leu862
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 194394146
• NCBI GeneID: 91039
• NCBI Accession #: NP_631898.3
• NCBI GenBank Nucleotide #: NM_139159.4
• Molecular Weight: 95,013 Da
• NCBI Official Full Name: dipeptidyl peptidase 9
• NCBI Official Synonym Full Names: dipeptidyl-peptidase 9
• NCBI Official Symbol: DPP9
• NCBI Official Synonym Symbols: DP9; DPLP9; DPRP2; DPRP-2
• NCBI Protein Information: dipeptidyl peptidase 9
• UniProt Protein Name: Dipeptidyl peptidase 9
• Protein Family: Dipeptidyl peptidase
• UniProt Gene Name: DPP9
• UniProt Synonym Gene Names: DPRP2; DP9; DPRP-2; DPP IX; DPLP9
• UniProt Entry Name: DPP9_HUMAN

NCBI Description

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP9: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Belongs to the peptidase S9B family. DPPIV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; serine-type peptidase activity; aminopeptidase activity

Biological Process: proteolysis