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Rabbit ETR1 Polyclonal Antibody | anti-ETR1 antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ETR1 Polyclonal Antibody

Gene Names
ETR1; AtETR1; EIN1; ETHYLENE INSENSITIVE 1; ETHYLENE RESPONSE; ETHYLENE RESPONSE 1; ETR; ETR1; HISTIDINE KINASE ETR1; T27F4.9; T27F4_9
Reactivity
Arabidopsis thaliana (Mouse-ear cress)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
ETR1; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ETR1 Polyclonal Antibody; ETR1 Antibody; Ethylene receptor 1; AtETR1; EC 2.7.13.3; Protein ETHYLENE RESPONSE 1; Protein ETR1; ETR1 At1g66340 T27F4.9; anti-ETR1 antibody
Ordering
Host
Rabbit
Reactivity
Arabidopsis thaliana (Mouse-ear cress)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-ETR1 antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Arabidopsis thaliana ETR1 protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,566 Da
NCBI Official Full Name
ethylene receptor 1
NCBI Official Symbol
ETR1
NCBI Official Synonym Symbols
AtETR1; EIN1; ETHYLENE INSENSITIVE 1; ETHYLENE RESPONSE; ETHYLENE RESPONSE 1; ETR; ETR1; HISTIDINE KINASE ETR1; T27F4.9; T27F4_9
NCBI Protein Information
ethylene receptor 1
UniProt Protein Name
Ethylene receptor 1
Protein Family
UniProt Gene Name
ETR1
UniProt Synonym Gene Names
AtETR1
UniProt Entry Name
ETR1_ARATH

NCBI Description

Similar to prokaryote sensory transduction proteins. Contains a histidine kinase and a response regulator domain. Homodimer. Membrane component. Binds ethylene. Mutations affect ethylene binding and metabolism of other plant hormones such as auxin, cytokinins, ABA and gibberellic acid. Ethylene receptor. Has histidine kinase activity. Is regulated by RTE1.

Uniprot Description

Function: Ethylene receptor related to bacterial two-component regulators. Acts as a redundant negative regulator of ethylene signaling. Ref.11 Ref.12

Catalytic activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactor: Binds 1 copper ion per dimer. Ref.7

Subunit structure: Homodimer; disulfide-linked. Heteromer with ERS1, ERS2, ETR2 and EIN4. Interacts with AHP1, AHP2 and AHP3. Interacts with RTE1. Ref.4 Ref.8 Ref.14 Ref.15

Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.9.

Tissue specificity: Leaves, roots, stems, seedlings, flowers, anthers, carpels and ovules. Ref.6

Domain: The GAF domain is sufficient to mediate heteromerization.

Post-translational modification: Autophosphorylated predominantly on His residues. Activation probably requires a transfer of a phosphate group between a His in the transmitter domain and an Asp of the receiver domain

By similarity. Ref.10

Disruption phenotype: No effect on ethylene response due to the redundancy with ERS1. Double mutants in ERS1 and ETR1 display a constitutive ethylene-response phenotype. Ref.13

Sequence similarities: Belongs to the ethylene receptor family.Contains 1 GAF domain.Contains 1 histidine kinase domain.Contains 1 response regulatory domain.

Research Articles on ETR1

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Product Notes

The ETR1 etr1 (Catalog #AAA7159832) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ETR1 Polyclonal Antibody reacts with Arabidopsis thaliana (Mouse-ear cress) and may cross-react with other species as described in the data sheet. AAA Biotech's ETR1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the ETR1 etr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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