Mouse Laminin Alpha 5 (LAMa5) ELISA Kit | LAMa5 elisa kit

Mouse Laminin Alpha 5 (LAMa5) ELISA Kit

Cat. Num. AAA2024735

• Reactivity: Mouse
• Synonyms: Laminin Alpha 5 (LAMa5); Mouse Laminin Alpha 5 (LAMa5) ELISA Kit; LAM-A5; LAMa5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Laminin Alpha 5 (LAMa5).; No significant cross-reactivity or interference between Laminin Alpha 5 (LAMa5) and analogues was observed.
• Sequence Length: 3690

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: < 33pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Alpha 5 (LAMa5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMa5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 5 (LAMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 5 (LAMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 5 (LAMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 5 (LAMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LAMa5 elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 119595780
• NCBI GeneID: 3911
• Molecular Weight: 61,287 Da
• NCBI Official Full Name: laminin, alpha 5, isoform CRA_c
• NCBI Official Synonym Full Names: laminin, alpha 5
• NCBI Official Symbol: LAMA5
• NCBI Protein Information: laminin subunit alpha-5
• UniProt Protein Name: Laminin subunit alpha-5
• Protein Family: Laminin
• UniProt Gene Name: LAMA5
• UniProt Synonym Gene Names: KIAA0533; KIAA1907
• UniProt Entry Name: LAMA5_HUMAN

NCBI Description

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Uniprot Description

LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 20q13.2-q13.3

Cellular Component: extracellular matrix; extracellular space; laminin-5 complex; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane; basal lamina; nucleus

Molecular Function: integrin binding; structural molecule activity

Biological Process: focal adhesion formation; extracellular matrix organization and biogenesis; muscle development; regulation of embryonic development; regulation of cell migration; extracellular matrix disassembly; morphogenesis of embryonic epithelium; hair follicle development; cilium biogenesis; angiogenesis; cell differentiation; neural crest cell migration; morphogenesis of a polarized epithelium; regulation of cell adhesion; integrin-mediated signaling pathway; endothelial cell differentiation; cell migration; cell recognition; cytoskeleton organization and biogenesis; regulation of cell proliferation; odontogenesis of dentine-containing teeth; cell proliferation; embryonic development; ureteric bud branching; lung development