Rabbit anti-Human ETNK2 Polyclonal Antibody | anti-ETNK2 antibody

ETNK2, NT (ETNK2, EKI2, Ethanolamine kinase 2, Ethanolamine kinase-like protein) (APC)

Cat. Num. AAA6296922

• Gene Names: ETNK2; EKI2; HMFT1716; RP11-74C13.2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ETNK2; Polyclonal Antibody; NT (ETNK2; EKI2; Ethanolamine kinase 2; Ethanolamine kinase-like protein) (APC); Ethanolamine kinase-like protein; anti-ETNK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ETNK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ETNK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the N-terminal region of human ETNK2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ETNK2 Antibody (N-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the ETNK2 antibody detected the ETNK2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of ETNK2 (arrow) using rabbit polyclonal ETNK2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ETNK2 gene.)

Related Product Information for anti-ETNK2 antibody

The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway.

Product Categories/Family for anti-ETNK2 antibody

Antibodies; Protein Kinases

References

Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009). Schoenhard, J.A., et al. Hum. Genet. 124(5):479-488(2008). Tian, Y., et al. J. Biol. Chem. 281(38):28438-28449(2006). Hurley, T.M., et al. Gene Expr. Patterns 4(2):135-140(2004). Lykidis, A., et al. J. Biol. Chem. 276(3):2174-2179(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 186659525
• NCBI GeneID: 55224
• NCBI Accession #: NP_060678.2
• NCBI GenBank Nucleotide #: NM_018208.2
• UniProt Accession #: Q9NVF9; Q5SXX5; Q68CK3; Q96G05; B7Z7K1
• Molecular Weight: 44,781 Da
• NCBI Official Full Name: ethanolamine kinase 2
• NCBI Official Synonym Full Names: ethanolamine kinase 2
• NCBI Official Symbol: ETNK2
• NCBI Official Synonym Symbols: EKI2; HMFT1716; RP11-74C13.2
• NCBI Protein Information: ethanolamine kinase 2; EKI 2; ethanolamine kinase-like protein
• UniProt Protein Name: Ethanolamine kinase 2
• Protein Family: Ethanolamine kinase
• UniProt Gene Name: ETNK2
• UniProt Synonym Gene Names: EKI2; EKI 2
• UniProt Entry Name: EKI2_HUMAN

NCBI Description

The protein encoded by this gene is a member of choline/ethanolamine kinase family which catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate (CDP) ethanolamine pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

ETNK2: Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity. Belongs to the choline/ethanolamine kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; EC 2.7.1.82; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytosol

Molecular Function: ethanolamine kinase activity; ATP binding

Biological Process: in utero embryonic development; phospholipid metabolic process; multicellular organism growth; phosphatidylethanolamine biosynthetic process; glycerophospholipid biosynthetic process; phosphorylation; placenta development; post-embryonic development