Rabbit anti-Human ESRP1 Polyclonal Antibody | anti-ESRP1 antibody

ESRP1, NT (ESRP1, RBM35A, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A, RNA-binding protein 35A) (FITC)

Cat. Num. AAA6296858

• Gene Names: ESRP1; RBM35A; RMB35A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ESRP1; Polyclonal Antibody; NT (ESRP1; RBM35A; Epithelial splicing regulatory protein 1; RNA-binding motif protein 35A; RNA-binding protein 35A) (FITC); RNA-binding protein 35A; anti-ESRP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ESRP1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ESRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-219 amino acids from the N-terminal region of human ESRP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ESRP1 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESRP1 antibody detected the ESRP1 protein (arrow).)

Immunohistochemistry (IHC)

(ESRP1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ESRP1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ESRP1 antibody

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Product Categories/Family for anti-ESRP1 antibody

Antibodies; RNA Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 170763529
• NCBI GeneID: 54845
• NCBI Accession #: NP_001116298.1; NP_060167.2; NP_001030087.2; NP_001116299.1; NP_001116297.1
• NCBI GenBank Nucleotide #: NM_001122826.1
• UniProt Accession #: Q6NXG1; Q2M2B0; Q499G3; Q6PJ86; Q9NXL8; A6NHA8; A8MPX1; E9PB47
• Molecular Weight: 73,133 Da
• NCBI Official Full Name: epithelial splicing regulatory protein 1 isoform 3
• NCBI Official Synonym Full Names: epithelial splicing regulatory protein 1
• NCBI Official Symbol: ESRP1
• NCBI Official Synonym Symbols: RBM35A; RMB35A
• NCBI Protein Information: epithelial splicing regulatory protein 1
• UniProt Protein Name: Epithelial splicing regulatory protein 1
• Protein Family: Epithelial splicing regulatory protein
• UniProt Gene Name: ESRP1
• UniProt Synonym Gene Names: RBM35A
• UniProt Entry Name: ESRP1_HUMAN

NCBI Description

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]

Uniprot Description

RBM35A: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleus

Molecular Function: mRNA binding; nucleotide binding

Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing