Rabbit anti-Human, Mouse ESR1 Polyclonal Antibody | anti-ESR1 antibody

ESR1 Polyclonal Antibody

Cat. Num. AAA125118

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ESR1; Polyclonal Antibody; ESR1 Polyclonal Antibody; Estrogen Receptor alpha: Estrogen Receptor a; DKFZp686N23123; ER; ESR; ESRA; Era; NR3A1; Estrogen Receptor alpha; anti-ESR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 595

• Applicable Applications for anti-ESR1 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 - 1:4000
• Species: Human
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human Estrogen Receptor alpha (NP_000116.2).
• Category: Primary antibody
• Immunogen: Recombinant protein of human ESR1
• Calculated Molecular Weight: 66kDa
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles..

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using Estrogen Receptor alpha antibody (MBS125118) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using ESR1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-ESR1 antibody

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.

Product Categories/Family for anti-ESR1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 544257
• NCBI GeneID: 2099
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: Observed MW: 75 kDa; Calculated MW: 35 kDa/47 kDa/ 53 kDa/ 66 kDa
• NCBI Official Full Name: Estrogen receptor
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); steroid hormone mediated signaling; gene expression

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance