Immunohistochemistry (IHC)
(MBS9216561 staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Rabbit anti-Human ERVK-7 Polyclonal Antibody | anti-ERVK-7 antibody
ERVK-7 Antibody (N-Term)
IHC-P~~1:25
Immunohistochemistry (IHC)
(MBS9216561 staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9216561 staining ERVK-7 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Western Blot (WB)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
HERV-K102: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Belongs to the beta type-B retroviral envelope protein family. HERV class-II K(HML-2) env subfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q22
Uniprot Description
HERV-K102: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Belongs to the beta type-B retroviral envelope protein family. HERV class-II K(HML-2) env subfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q22
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Product Notes
The ERVK-7 ervk-7 (Catalog #AAA9216561) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERVK-7 Antibody (N-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERVK-7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA, IHC-P. WB~~:1000-1:2000 IHC-P~~1:25. Researchers should empirically determine the suitability of the ERVK-7 ervk-7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERVK-7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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