Rabbit anti-Human ERP29 Polyclonal Antibody | anti-ERP29 antibody

ERP29, ID (Endoplasmic Reticulum Resident Protein 29, Endoplasmic Reticulum Resident Protein 31, Endoplasmic Reticulum Resident Protein 28, ERp31, ERp28, C12orf8) (PE)

Cat. Num. AAA6473936

• Gene Names: ERP29; ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ERP29; Polyclonal Antibody; ID (Endoplasmic Reticulum Resident Protein 29; Endoplasmic Reticulum Resident Protein 31; Endoplasmic Reticulum Resident Protein 28; ERp31; ERp28; C12orf8) (PE); anti-ERP29 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERP29.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ERP29 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 151-179 in the central region of human ERP29, UniProt Accession #: P30040.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS626505 and cell line lysates (35ug/lane) Lane 1: A2058 Lane 2: A375 Lane 3: MCF7 Lane 4: NCI-H460 Lane 5: Y79 The ERP29 protein(arrow) was detected.)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS626505 either nontransfected (Lane 1) or transiently transfected with the ERP29 gene (Lane 2))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human hepatocarcinoma tissue using MBS626505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS626505 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H292 cells using MBS626505 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ERP29 antibody

Antibodies; Signal Transduction

References

1. Bambang, I.F., et.al., Exp. Cell Res. 315(11), 1964-1974 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5803013
• NCBI GeneID: 10961
• NCBI Accession #: NP_006808
• NCBI GenBank Nucleotide #: NM_006817
• UniProt Accession #: P30040; Q3MJC3; Q6FHT4; C9J183
• Molecular Weight: 5,477 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 29 isoform 1
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 29
• NCBI Official Symbol: ERP29
• NCBI Official Synonym Symbols: ERp28; ERp31; PDIA9; PDI-DB; C12orf8; HEL-S-107
• NCBI Protein Information: endoplasmic reticulum resident protein 29; endoplasmic reticulum lumenal protein ERp28; endoplasmic reticulum resident protein 28; endoplasmic reticulum resident protein 31; epididymis secretory protein Li 107; protein disulfide isomerase family A, member
• UniProt Protein Name: Endoplasmic reticulum resident protein 29
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP29
• UniProt Synonym Gene Names: C12orf8; ERP28; ERp29; ERp28; ERp31
• UniProt Entry Name: ERP29_HUMAN