Rabbit anti-Human, Mouse ERO1LB Polyclonal Antibody | anti-ERO1LB antibody

ERO1LB, CT (ERO1LB, ERO1-like protein beta, Endoplasmic oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta)

Cat. Num. AAA6001250

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ERO1LB; Polyclonal Antibody; CT (ERO1LB; ERO1-like protein beta; Endoplasmic oxidoreductin-1-like protein B; Oxidoreductin-1-L-beta); Anti -ERO1LB; anti-ERO1LB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ERO1LB antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: ERO1LB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the C-terminal region of human ERO1LB.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB435 cell line lysates using MBS6001250 (35ug/lane). This demonstrates MBS6001250 detected the ERO1LB protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue using MBS6001250 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001250 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB435 cells using MBS6001250 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ERO1LB antibody

ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.

Product Categories/Family for anti-ERO1LB antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 239582761
• NCBI GeneID: 56605
• NCBI Accession #: NP_063944.3
• NCBI GenBank Nucleotide #: NM_019891.3
• UniProt Accession #: Q86YB8; Q5T1H4; Q8IZ11; Q9NR62
• Molecular Weight: 53,543 Da
• NCBI Official Full Name: ERO1-like protein beta
• NCBI Official Synonym Full Names: ERO1-like beta (S. cerevisiae)
• NCBI Official Symbol: ERO1LB
• NCBI Protein Information: ERO1-like protein beta; ERO1-L-beta; oxidoreductin-1-L-beta; endoplasmic oxidoreductin-1-like protein B
• UniProt Protein Name: ERO1-like protein beta
• UniProt Gene Name: ERO1LB
• UniProt Synonym Gene Names: ERO1-L-beta
• UniProt Entry Name: ERO1B_HUMAN

Uniprot Description

ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family.

Protein type: Oxidoreductase; Endoplasmic reticulum; EC 1.8.4.-

Chromosomal Location of Human Ortholog: 1q42.2-q43

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum

Molecular Function: protein binding; unfolded protein binding; protein disulfide oxidoreductase activity; protein disulfide isomerase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: insulin processing; extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; protein folding; cell redox homeostasis; glucose homeostasis