Rabbit ERK5 Polyclonal Antibody | anti-MAPK7 antibody

ERK5, phosphorylated (Thr218, Tyr220) (Extracellular Signal-regulated Kinase 5, ERK-5, Big MAP Kinase 1, BMK1, BMK-1, Mitogen-activated Protein Kinase 7, MAP Kinase 7, MAPK 7, MAPK7, PRKM7) (BSA & Azide Free)

Cat. Num. AAA613080

• Gene Names: MAPK7; BMK1; ERK4; ERK5; PRKM7
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by protein A and immunoaffinity chromatography.
• Synonyms: ERK5; Polyclonal Antibody; phosphorylated (Thr218; Tyr220) (Extracellular Signal-regulated Kinase 5; ERK-5; Big MAP Kinase 1; BMK1; BMK-1; Mitogen-activated Protein Kinase 7; MAP Kinase 7; MAPK 7; MAPK7; PRKM7) (BSA & Azide Free); Anti -ERK5; anti-MAPK7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of Erk5 phosphorylated at threonine 218/tyrosine 220. This antibody crossreacts with phosphorylated Erk1 and Erk2. It does not crossreact with phosphorylated p38 MAPK or SAPK/JNK.
• Purity/Purification: Affinity Purified; Purified by protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Also available with BSA & Azide. See E3452-59A.

• Applicable Applications for anti-MAPK7 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr218/Tyr220 of human Erk5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PC12 cells overexpressing TrkA, untreated or treated with NGF and BDNF. using Phospho-Erk5 (Thr218/Tyr220) Antibody. (Provided by Dr. R.A. Segal, Dept. of Neurobiology, Harvard Medical School, Massachusetts.))

Western Blot (WB)

(Western blot analysis of extracts from HEK293 cells, untreated, EGF-treated or In1B-treated, using Phospho-Erk5 (Thr218/Tyr220) Antibody.)

Related Product Information for anti-MAPK7 antibody

Erk5, also known as BMK1 (big mitogen-activated kinase 1), has a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the N-terminal kinase domain with Erk1/2. However, the long C-terminal domain makes Erk5 a unique MAP kinase. Erk5 is activated by a wide variety of extracellular signals, including tyrosine receptor kinases, G-protein-coupled receptors as well as osmotic and oxidative stress (1,2). The direct upstream kinase of Erk5 is defined as MEK5 (1). MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3-5). Growing evidence indicates that the Erk5 pathway is involved in proliferation/differentiation regulation, oncogenesis and neuronal development (1,6-8).

Product Categories/Family for anti-MAPK7 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 837261
• NCBI GeneID: 5598
• UniProt Accession #: Q13164; Q16634; Q59F50; Q6QLU7; Q7L4P4; Q969G1; Q96G51
• Molecular Weight: 88,386 Da
• NCBI Official Full Name: ERK5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 7
• NCBI Official Symbol: MAPK7
• NCBI Official Synonym Symbols: BMK1; ERK4; ERK5; PRKM7
• NCBI Protein Information: mitogen-activated protein kinase 7; BMK-1; ERK-5; MAPK 7; BMK1 kinase; MAP kinase 7; big MAP kinase 1; OTTHUMP00000065906; OTTHUMP00000065907; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase 7
• UniProt Gene Name: MAPK7
• UniProt Synonym Gene Names: BMK1; ERK5; PRKM7
• UniProt Entry Name: MK07_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq]

Uniprot Description

ERK5: a protein kinase of the MAPK family. Phosphorylated and activated by MEK5. Involved in the signaling processes downstream of various receptors including receptor tyrosine kinases and G protein-coupled receptors. Translocates to cell nucleus in response to extracelluar signals, where it regulates gene expression by phosphorylating various transcription factors. Mouse knockout shows a role in development and integrity of blood vessels. May block apoptosis in endothelial cells. Required for transduction of EGF growth signal, constitutively activated in ErbB2-overexpressing breast cancer, and downstream of activated Mek5 in metastatic prostate cancerFour alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; PML body; cytoplasm; nucleus; cytosol

Molecular Function: MAP kinase activity; protein binding; mitogen-activated protein kinase binding; ATP binding

Biological Process: negative regulation of cAMP catabolic process; nerve growth factor receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; positive regulation of protein metabolic process; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cAMP-mediated signaling; cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of heterotypic cell-cell adhesion; MyD88-independent toll-like receptor signaling pathway; negative regulation of NFAT protein import into nucleus; cell cycle; regulation of angiogenesis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of cyclic-nucleotide phosphodiesterase activity