Rabbit anti-Human ERF Polyclonal Antibody | anti-ERF antibody

ERF, CT (ERF, ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE-2)

Cat. Num. AAA641409

• Gene Names: ERF; PE2; CRS4; PE-2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ERF; Polyclonal Antibody; CT (ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE-2); Anti -ERF; anti-ERF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ERF antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ERF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 485-514 amino acids from the C-terminal region of human ERF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS641409. This demonstrates that MBS641409 detected the ERF protein (arrow).)

Related Product Information for anti-ERF antibody

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).

Product Categories/Family for anti-ERF antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 156104872
• NCBI GeneID: 2077
• NCBI Accession #: NP_006485.2
• NCBI GenBank Nucleotide #: NM_006494.2
• UniProt Accession #: P50548; Q59G38; Q9UPI7; B2RAP1
• Molecular Weight: 58,703 Da
• NCBI Official Full Name: ETS domain-containing transcription factor ERF
• NCBI Official Synonym Full Names: Ets2 repressor factor
• NCBI Official Symbol: ERF
• NCBI Official Synonym Symbols: PE2; CRS4; PE-2
• NCBI Protein Information: ETS domain-containing transcription factor ERF
• UniProt Protein Name: ETS domain-containing transcription factor ERF
• Protein Family: eRF1 methyltransferase
• UniProt Gene Name: ERF
• UniProt Entry Name: ERF_HUMAN

NCBI Description

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM, Mar 2008]

Uniprot Description

ERF: potent transcriptional repressor that binds to the H1 element of the ETS2 promoter. Belongs to the ETS family of transcription factors. Its function is regulated by phosphorylation.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; nucleus; cytosol

Molecular Function: sequence-specific DNA binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; cell cycle

Disease: Craniosynostosis 4