Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat ERBB2/HER2 Polyclonal Antibody | anti-ERBB2 antibody
Goat anti-ERBB2/HER2 (aa182-194) Antibody
Immunohistochemistry: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration, 4-6ug/ml.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human breast cancer (Her+ left, triple negative right). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.
Protein type: EC 2.7.10.1; Oncoprotein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; EGFR family
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: apical plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; integral to membrane; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; receptor complex
Molecular Function: ATP binding; ErbB-3 class receptor binding; growth factor binding; identical protein binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein C-terminus binding; protein dimerization activity; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: cell proliferation; cell surface receptor linked signal transduction; enzyme linked receptor protein signaling pathway; heart development; MAPKKK cascade; motor axon guidance; myelination; negative regulation of immature T cell proliferation in the thymus; neuromuscular junction development; oligodendrocyte differentiation; peptidyl-tyrosine phosphorylation; peripheral nervous system development; phosphoinositide 3-kinase cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of angiogenesis; regulation of microtubule-based process; regulation of phosphoinositide 3-kinase cascade; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing
Disease: Gastric Cancer; Glioma Susceptibility 1; Lung Cancer
Research Articles on ERBB2
Similar Products
Product Notes
The ERBB2 erbb2 (Catalog #AAA423325) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ERBB2/HER2 (aa182-194) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERBB2/HER2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration, 4-6ug/ml. Researchers should empirically determine the suitability of the ERBB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TLIDTNRSRA CHP. It is sometimes possible for the material contained within the vial of "ERBB2/HER2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
