Rabbit anti-Human ERAP2 Polyclonal Antibody | anti-ERAP2 antibody

ERAP2 Antibody

Cat. Num. AAA153649

• Gene Names: ERAP2; LRAP; L-RAP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: ERAP2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ERAP2; Polyclonal Antibody; ERAP2 Antibody; ERAP2 Antibody : LRAP; L-RAP; LRAP; Endoplasmic reticulum aminopeptidase 2; Leukocyte-derived arginine aminopeptidase; endoplasmic reticulum aminopeptidase 2; anti-ERAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ERAP2 antibody is human reactive. Multiple isoforms of ERAP2 are known to exist. ERAP2 antibody is predicted to not cross-react with ERAP1.
• Purity/Purification: ERAP2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 960

• Applicable Applications for anti-ERAP2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ERAP2 antibody can be used for detection of ERAP2 by Western blot at 1 - 2 mug/ml.
• Conjugate: Unconjugated
• Immunogen: ERAP2 antibody was raised against a 17 amino acid peptide near the amino terminus of human ERAP2.
• Buffer: ERAP2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ERAP2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of ERAP2 in human ovary tissue lysate with ERAP2 antibody at 1 μg/ml.)

Related Product Information for anti-ERAP2 antibody

The endoplasmic reticulum (ER) aminopeptidase 2 (ERAP2), a member of the peptidase M1 family, like the related protein ERAP1, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). Like ERAP1, ERAP2 is localized to the lumen of the ER and is thought to associate with ERAP1 as a heterodimer (1). Both ERAP1 and ERAP2 have been linked to several human diseases ranging from infections to autoimmunity and cancer, and may play a role in the innate immune response (reviewed in 3).

NCBI and Uniprot Product Information

• NCBI GI #: 11641261
• NCBI GeneID: 64167
• NCBI Accession #: NP_071745
• NCBI GenBank Nucleotide #: NM_022350.3
• UniProt Accession #: Q6P179; Q7Z5K1; Q8TD32; Q8WVJ4; Q9HBX2
• Molecular Weight: Predicted: 106 kDa; ; Observed: 120kDa
• NCBI Official Full Name: endoplasmic reticulum aminopeptidase 2
• NCBI Official Synonym Full Names: endoplasmic reticulum aminopeptidase 2
• NCBI Official Symbol: ERAP2
• NCBI Official Synonym Symbols: LRAP; L-RAP
• NCBI Protein Information: endoplasmic reticulum aminopeptidase 2; leukocyte-derived arginine aminopeptidase
• UniProt Protein Name: Endoplasmic reticulum aminopeptidase 2
• Protein Family: Endoplasmic reticulum aminopeptidase
• UniProt Gene Name: ERAP2
• UniProt Synonym Gene Names: LRAP; L-RAP
• UniProt Entry Name: ERAP2_HUMAN

NCBI Description

Aminopeptidases hydrolyze N-terminal amino acids of proteins or peptide substrates. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP1 (MIM 606832) and LRAP to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II (TPP2; MIM 190470) (Tanioka et al., 2003 [PubMed 12799365]).[supplied by OMIM, Mar 2008]

Uniprot Description

ERAP2: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys. Belongs to the peptidase M1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.11.-; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen; integral to membrane

Molecular Function: metallopeptidase activity; zinc ion binding; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of blood pressure; proteolysis; antigen processing and presentation of endogenous peptide antigen via MHC class I