Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat EPS8 Polyclonal Antibody | anti-EPS8 antibody
Goat anti-EPS8 Antibody
Immunohistochemistry: In paraffin embedded Human Breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1ug/ml.
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
EPS8: an adaptor protein that is an epidermal growth factor receptor (EGFR) substrate. Enhances EGF-dependent mitogenic signals. Member of a family of actin barbing and capping proteins. Promotes filopodial protrusions. Complexes containing Eps8/Abi1/PI3K/Sos-1 mediate Rac activation following EGFR signaling. Involved in endocytosis of EGFR by binding to the Rab5-specific GTPase activating protein USP6N. Binds to IRSp53, which binds small the small GTPases Rac and Cdc42, and which regulates actin dynamics. Involved in the actin-remodeling activity in cultured cerebellar neurons. Regulates the actin-remodeling activities of NMDA and ethanol in neurons. Eps8 knockout mice show behavioral resistance to ethanol. Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 12p12.3
Cellular Component: brush border; cell cortex; cell junction; growth cone; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic density; stereocilium; vesicle
Molecular Function: actin binding; protein binding; Rac GTPase binding; SH3/SH2 adaptor activity
Biological Process: actin crosslink formation; actin cytoskeleton reorganization; actin filament bundle formation; adult locomotory behavior; barbed-end actin filament capping; behavioral response to ethanol; cell proliferation; epidermal growth factor receptor signaling pathway; exit from mitosis; positive regulation of signal transduction; Rac protein signal transduction; regulation of actin filament length; regulation of cell shape; signal transduction
Disease: Deafness, Autosomal Recessive 102
Research Articles on EPS8
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Product Notes
The EPS8 eps8 (Catalog #AAA420017) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EPS8 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's EPS8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe. Recommended concentration, 5-10ug/ml. Western Blot: Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1ug/ml. Researchers should empirically determine the suitability of the EPS8 eps8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SGVESFDEGS SH. It is sometimes possible for the material contained within the vial of "EPS8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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